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Detection and estimation of vitamin D2 in catharanthus roseus by HPLC and other molecular spectra instruments as a source of vtamin D3 production
Joint Authors
Awdah, Mufid Jalil
Muhammad, Nisrin Jalal
Ayyash, Muhammad Abbud
Source
Issue
Vol. 13, Issue 2 (30 Jun. 2016), pp.468-480, 13 p.
Publisher
University of Babylon College of Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
Vitamin D is one of fat-soluble vitamins, considered a steroid hormone.
It is regulate and responsible for improving the metabolism and absorption of calcium, iron, magnesium, zinc, and phosphate in small intestine.
SHIMADZU High-Performance Liquid Chromatography was used for qualitative and quantitative estimation of vitamin D2 in aqueous extracted of Catharanthus Roseus plant; (20μl, 2.5 IU/gm) standard material of pure vitamin D2 (Ergocalciferol) injected and measured at reference conditions to fix retention time (RT), then 20μlof extracted plant sample was injected and measured at the same conditions.
An intensive analysis conducted on aqueous, alcoholic extract and dry powder of plant leaves using modern and sophisticated instruments.
Quantitative results from HPLC shows thatVit.D2 in drying weight of plant up to 187.840 IU/gm.
Qualitative results from HPLC, UV-VIS, IR, ATR-FTIR shows match the effective groups of pure standard Vit.D2 and alcoholic extract, dry powder of C.
Roseus plant.
This study aimed to investigate the presence of vitamin D2 in C.
Roseus, in addition to qualitative and quantitative detection in aqueous, alcoholic extract, and dry powder of plant leavesto use it in practical as a major source for Vit.
D3 production in industrial scale; in order to use it in the medical, pharmaceutical and what is called alternative medicine (Herbs) fields.
American Psychological Association (APA)
Ayyash, Muhammad Abbud& Awdah, Mufid Jalil& Muhammad, Nisrin Jalal. 2016. Detection and estimation of vitamin D2 in catharanthus roseus by HPLC and other molecular spectra instruments as a source of vtamin D3 production. Medical Journal of Babylon،Vol. 13, no. 2, pp.468-480.
https://search.emarefa.net/detail/BIM-750662
Modern Language Association (MLA)
Ayyash, Muhammad Abbud…[et al.]. Detection and estimation of vitamin D2 in catharanthus roseus by HPLC and other molecular spectra instruments as a source of vtamin D3 production. Medical Journal of Babylon Vol. 13, no. 2 (2016), pp.468-480.
https://search.emarefa.net/detail/BIM-750662
American Medical Association (AMA)
Ayyash, Muhammad Abbud& Awdah, Mufid Jalil& Muhammad, Nisrin Jalal. Detection and estimation of vitamin D2 in catharanthus roseus by HPLC and other molecular spectra instruments as a source of vtamin D3 production. Medical Journal of Babylon. 2016. Vol. 13, no. 2, pp.468-480.
https://search.emarefa.net/detail/BIM-750662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 479-480
Record ID
BIM-750662