تأثير تنشيط الحيامن و إضافة السائل الحويصلي المعامل للأغنام في تحسين بعض صفات السائل المنوي المجمد للكباش العواسية
Other Title(s)
Effect of sperm activation and addition of treated sheep follicular fluid on the quality of Awassi cryopreserved semen
Joint Authors
السعدون، علي عبد الله زعيري
الصعب، حازم كسار جاسر
كاطع، إقبال عوض
Source
Issue
Vol. 8, Issue 4 (31 Dec. 2016), pp.184-192, 9 p.
Publisher
University of Kufa Faculty of Agriculture
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
This study was conducted at the laboratory of Higher study /Animal Resources Department /College of Agriculture /University of Al-Muthanna at the summer of 2015, to evaluate the effect of follicle fluid to improvement of fertilization ability (representing on individual motility and progressive motility) of ram sperms after cryopreservation- thawing .
Twenty five cryo-semen straw for four Awassi rams were thawed gradually and evaluated initially for individual motility and progressive motility, than the semen was divided into two major part, part one was divided into four eppendrof tubes and extended with SMART medium treated with follicular fluid (FF) in concentrations with (0, 5, 10, 15%) , semen from this four treatment was evaluated for individual motility and progressive motility to understand the effect of FF on this characteristics'.
Part two also divided into four eppendrof tubes and subjected into sperm activation using swim-up technique and extending with SMART medium treated with follicular fluid (FF) in concentrations with (0, 5,10,15%) and incubated under 37C˚ .After 45 minutes ,the four treatment was evaluated for individual motility and progressive motility to learning the effect of sperm activation with FF on improvement this characteristics as a model for semen quality .
The results indicated that the activation was significantly (P<0.05) improve the individual motility(11.67±0.09) after activation as compared with (5.00±0.03) before activation , while the activation was highly significantly (P<0.01) improve the individual motility (30.00±0.27 and 41.67±0.32) and progressive motility (13.33±0.07 and 31.67± 0.23) of ram sperms with highest concentration (10,15%) of FF.
According the result of the present study , there was an ability to improve the quality of Awassi ram semen after cryopreservation –thawing by applying the sperm activation (swim-up technique) with SMART medium treated with (10,15%) of FF before Artificial Insemination.
American Psychological Association (APA)
السعدون، علي عبد الله زعيري والصعب، حازم كسار جاسر وكاطع، إقبال عوض. 2016. تأثير تنشيط الحيامن و إضافة السائل الحويصلي المعامل للأغنام في تحسين بعض صفات السائل المنوي المجمد للكباش العواسية. مجلة الكوفة للعلوم الزراعية،مج. 8، ع. 4، ص ص. 184-192.
https://search.emarefa.net/detail/BIM-750681
Modern Language Association (MLA)
السعدون، علي عبد الله زعيري....[و آخرون]. تأثير تنشيط الحيامن و إضافة السائل الحويصلي المعامل للأغنام في تحسين بعض صفات السائل المنوي المجمد للكباش العواسية. مجلة الكوفة للعلوم الزراعية مج. 8، ع. 4 (2016)، ص ص. 184-192.
https://search.emarefa.net/detail/BIM-750681
American Medical Association (AMA)
السعدون، علي عبد الله زعيري والصعب، حازم كسار جاسر وكاطع، إقبال عوض. تأثير تنشيط الحيامن و إضافة السائل الحويصلي المعامل للأغنام في تحسين بعض صفات السائل المنوي المجمد للكباش العواسية. مجلة الكوفة للعلوم الزراعية. 2016. مج. 8، ع. 4، ص ص. 184-192.
https://search.emarefa.net/detail/BIM-750681
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 189-190
Record ID
BIM-750681