Isolation and identification of Salmonella spp. from chicken and chicken environment in Basrah Province

Abstract EN

This study was designated to investigate the distribution of Salmonella in Basrah province through bacteriological examination for different types of samples at different ages and to detect zoonotic serotypes of Salmonella by Polymerase Chain Reaction assay.

The first part of the study included 370 collected samples, 50 samples for each of liver, yolk sac and cecal content, 100 cloacal swabs, 30 samples of poultry litter, 30 samples of poultry ration and 60 samples of embryonated egg.

Results showed significant differences (P< 0.01) among different types of collected samples; cloacal swabs exhibited high percentage of isolation (19%).

The results obtained showed that no significant differences among the broiler and layer cloacal swabs.

Three hundred and thirty broiler samples were collected from Al-Hartha, Garmat Ali, Al-Zubair and Abu Al-Khaseeb, 30 out of these total samples were positive for Salmonella (9.09%).

Significant differences (P< 0.01) were found among the studied locations and the highest isolation rate was at Al-Zubair (14.47%).

While 40 layer cloacal swab samples were collected from local markets at Garmat Ali, Al-Ashaar, Basrah Al-kadima, and Khmsa mile, 4 samples were positive for Salmonella (10%).

The results also showed that the highest rate of isolation was at the 2nd week of age with high significance difference (P<0.01) among ages.

S.

typhimurium, S.

emek, S.

anatum, S.

goodwood and S.

enteritidis were the main common serovars among 34 Salmonella isolates after serotyping and PCR assay.

The second part of the study included detection of S.

typhimurium and S.

enteritidis by PCR technique with specific primers for fimC and sefA genes respectively.

DNA was extracted for 34 isolates, 14 isolates was detected as S.

typhimurium and 2 isolates was detected as S.

enteritidis at 289bp and 330bp after electrophoresis respectively