Identification of some meat species using PCR and multiplex PCR of mitochondrial cytochrome B gene

Abstract EN

The objective of this study was the designing of a fast and reliable multiplex polymerase chain reaction (PCR) identification system for testing the pure species origin of meat samples.

The present study involved the application of molecular biological approach to identify and differentiate some meat species i.e.

cattle, buffalo, sheep, goat, chicken, horse and pig.

The DNA was isolated from meat samples of each species and a part of the mitochondrial cytochrome b gene was amplified through PCR.

Different lengths of specific PCR products were detected to be 157,227,274,274,331,398 and 439 bp for goat, chicken, cattle, buffalo, sheep, pig and horse, respectively.

Also, the identification of all meat species studied was determined by multiplex PCR using species-specific primers on mitochondrial cytochrome b gene.

The results of the present study demonstrate that multiplex PCR is characterized by high efficiency and sensitivity even with very small amounts of fresh meat.

It is concluded that multiplex PCR can be useful for fast, easy, and reliable control of adulterated consumer meat products