تأثير منظمات النمو النباتية في تحفيز إنتاج مضادات الأكسدة النباتية من كالس نبات الحنطة السوداء

Abstract EN

This experiment was conducted during 2012 and 2013 at the tissue culture Laboratory, Date Palm Research Unite, College of Agriculture, University of Baghdad.

The objectives of this study effect of plant growth regulators in the production of anti-oxidants in callus of buckwheat Fagopyrum esculentum M.

It was found that the best concentration of NaOCl was %4.5 for seeds sterilization for 20 minutes (%90).

The best plant growth regulater combination for callus induction from hypocotyl of Buckwheat seedling was 2,4-D and Kinetin at 3.0 and 1.0 mg.L-1 respectively, was 433.2 and 34.61 mg.

respectively.

The highest values of fresh and dry weights of callus was achieved at 1.0 mg.L-1 of salicylic acid was 349.4 and 27.7 mg respectively.

Results indicated the concentration of Brassinolide 0.050 mg.L-¹ increased Caffeic acid and Vanillic acid.

While 0.10 mg.L-¹ of Brassinolide increased Chlorogenic acid, Orientin, Vitexin and Rutin.

Also 2.0 mg.L-¹ of salicylic acid increased Gentisic acid, Isovitexin, Quercetin and Coumaric acid.