Novel methods to design wild bacteriophages into highly lytic and therapeutic bacteriophages to extensively drug-resistant mycobacterium tuberculosis
Author
Source
Journal of the Faculty of Medicine Baghdad
Issue
Vol. 58, Issue 3 (30 Sep. 2016), pp.276-282, 7 p.
Publisher
University of Baghdad Faculty of Medicine
Publication Date
2016-09-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Background: The emergence of multiple drug resistant (MDR) Mycobacterium tuberculosis (M.TB) and extensive drug resistant (XDR) M.TB lay huge burden on TB endemic countries such as Iraq.
Objectives: Bacteriophage (phage) therapy can be used as alternative approach to tackle this problem.
Patients and methods: Forty isolates of M.TB were cultured from TB-positive sputum specimens with three ATCC strains.
Phage passaging and biokinetic based techniques were used to optimize wild anti-M.TB phages.
Three chemical, non genetic- designing techniques, tween-80, mycobacterial lysis buffer, and xyelen, were used to change the specificity of wild anti-TB phages towards phage-resistant target M.TB.
Results: Five wild anti-M.TB phages were isolated and optimized.
The optimization techniques were successful in enhancing plaques size, clarity, burst size, and infective ratio.
Chemical designing techniques succeeded to reorient specificity of 6 phages to new host bacteria.
Conclusions: phage designing opens door wide for endless future applications of phage-based therapy, biocontrol and diagnosis
American Psychological Association (APA)
Abd al-Amir, Ahmad S.. 2016. Novel methods to design wild bacteriophages into highly lytic and therapeutic bacteriophages to extensively drug-resistant mycobacterium tuberculosis. Journal of the Faculty of Medicine Baghdad،Vol. 58, no. 3, pp.276-282.
https://search.emarefa.net/detail/BIM-753052
Modern Language Association (MLA)
Abd al-Amir, Ahmad S.. Novel methods to design wild bacteriophages into highly lytic and therapeutic bacteriophages to extensively drug-resistant mycobacterium tuberculosis. Journal of the Faculty of Medicine Baghdad Vol. 58, no. 3 (2016), pp.276-282.
https://search.emarefa.net/detail/BIM-753052
American Medical Association (AMA)
Abd al-Amir, Ahmad S.. Novel methods to design wild bacteriophages into highly lytic and therapeutic bacteriophages to extensively drug-resistant mycobacterium tuberculosis. Journal of the Faculty of Medicine Baghdad. 2016. Vol. 58, no. 3, pp.276-282.
https://search.emarefa.net/detail/BIM-753052
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 281-285
Record ID
BIM-753052