Recent advances in diagnosis of flavobacteriosis among some freshwater fishes
Joint Authors
Isa, I. A. M.
Ismail, Muna Mahmud
Dirwah, H. I.
al-Lami, Mathir M. M.
al-Sayyid, T. M.
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 20, Issue 2 (31 Dec. 2015), pp.31-42, 12 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Columnaris disease caused by Flavobacterium columnare exists worldwide and affects a wide variety of fishes.
This study aimed to investigate the isolation and characterization of Flavobacterium columnare from naturally infected fishes (Oreochromis niloticus and Clarias gariepinus) in Ismailia, using conventional biochemical methods and molecular techniques.
Bacteriological examination revealed presence of 162 suspected Cytophaga isolates of which only 102 isolates were characterized as F.
columnare strains according to colony character on Cytophaga agar which appeared yellow or whitish yellow in color, flat irregular with stiletto edge, clear swarming and rhizoid characters.
Biochemical characterizations demonstrated that all isolates tested uniformly positive for catalase, gelatin liquefaction, absorption of Congo Red and flexirubin pigment, but they were negative for indol, Methyl red, Vogus Proskauer, and lactose, glucose, saccharose, manitol, arabinose and maltose fermentation.
All isolates produced unique and clear PCR bands of the expected size (675 base pair).
Consequently, PCR confirmed the positive results of the biochemical reactions of suspected isolated strain and concluded that it is of F.columnare.
American Psychological Association (APA)
Isa, I. A. M.& Dirwah, H. I.& Ismail, Muna Mahmud& al-Lami, Mathir M. M.& al-Sayyid, T. M.. 2015. Recent advances in diagnosis of flavobacteriosis among some freshwater fishes. Suez Canal Veterinary Medicine Journal،Vol. 20, no. 2, pp.31-42.
https://search.emarefa.net/detail/BIM-753909
Modern Language Association (MLA)
Isa, I. A. M.…[et al.]. Recent advances in diagnosis of flavobacteriosis among some freshwater fishes. Suez Canal Veterinary Medicine Journal Vol. 20, no. 2 (2015), pp.31-42.
https://search.emarefa.net/detail/BIM-753909
American Medical Association (AMA)
Isa, I. A. M.& Dirwah, H. I.& Ismail, Muna Mahmud& al-Lami, Mathir M. M.& al-Sayyid, T. M.. Recent advances in diagnosis of flavobacteriosis among some freshwater fishes. Suez Canal Veterinary Medicine Journal. 2015. Vol. 20, no. 2, pp.31-42.
https://search.emarefa.net/detail/BIM-753909
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 39-41
Record ID
BIM-753909