Genotypic characterization of Clostridium Perfringens and associated Histopathological changes in broiler
Other Title(s)
التوصيف الجيني للكولستريديم بيرفيرنجينز و التغيرات الهستوباثولوجية للنزلات المعوية التنكرزية في بداري التسمين
Joint Authors
Afifi, Muna Maghawiri
Abd al-Rahman, Nuha Jamal
Badr, Suhayr Ahmad
Muhammad, Suhayr Yusuf
Anani, Muhammad al-Sayyid
Ammar, Ahmad Muhammad Karam
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 20, Issue 2 (31 Dec. 2015), pp.439-447, 9 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In the present study, 6 C.perfringens strains (4 were type A and 2 were type D) were recovered from intestinal and liver samples of 6 diseased broiler.
PCR used for the detection of alpha and epsilon toxin .
Multiplex PCR was done to C.perfringens isolates which had the gene gave a characteristic` band at 402 bp and 541 bp where all of the 6 isolates were positive for alpha toxin genes.The extracted toxins were purified by ammonium sulfate precipitation, then profiling of the exotoxin proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE).SDS-PAGE has become an important tool for protein profiling which reflect the genetic identity to C.perfringense type A and D.
Our study revealed the histopathological changes of intestinal samples of the diseased birds with necrotic enteritis showed Intestine hyaline degeneration of the muscle, fragmentation and edema in between the muscle infiltrated with inflammatory cells.
Liver showing fibrous connective tissue in the portal area particularly around intrahepatic bile duct and hyperplastic proliferation of biliary epithelium toward the lumen associated with central vein
American Psychological Association (APA)
Anani, Muhammad al-Sayyid& Ammar, Ahmad Muhammad Karam& Afifi, Muna Maghawiri& Abd al-Rahman, Nuha Jamal& Badr, Suhayr Ahmad& Muhammad, Suhayr Yusuf. 2015. Genotypic characterization of Clostridium Perfringens and associated Histopathological changes in broiler. Suez Canal Veterinary Medicine Journal،Vol. 20, no. 2, pp.439-447.
https://search.emarefa.net/detail/BIM-754232
Modern Language Association (MLA)
Anani, Muhammad al-Sayyid…[et al.]. Genotypic characterization of Clostridium Perfringens and associated Histopathological changes in broiler. Suez Canal Veterinary Medicine Journal Vol. 20, no. 2 (2015), pp.439-447.
https://search.emarefa.net/detail/BIM-754232
American Medical Association (AMA)
Anani, Muhammad al-Sayyid& Ammar, Ahmad Muhammad Karam& Afifi, Muna Maghawiri& Abd al-Rahman, Nuha Jamal& Badr, Suhayr Ahmad& Muhammad, Suhayr Yusuf. Genotypic characterization of Clostridium Perfringens and associated Histopathological changes in broiler. Suez Canal Veterinary Medicine Journal. 2015. Vol. 20, no. 2, pp.439-447.
https://search.emarefa.net/detail/BIM-754232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 445-447
Record ID
BIM-754232