Effect of obesity on serum estrogen concentration and ovarian growth with uterine development in mature female Wistar rats

Abstract EN

The present study has been carried out at the College of Veterinary Medicine ,AL-Qadisiya University to determine the effect of obesity on serum Estrogen concentration in mature female Wistar rats.Fifty immature female rats (14-20 days age,30-40g.) were housed at the animal house during the period extended from 25th March,2011 to 10th June,2011.Animals were randomly divided into two equal groups.

Control fed on standard feed and treated fed on high fat feed ( 32.3%fat by weight and 4828 k cal/kg energy).

Daily body weights were recorded during the experiment period extended to the 100th day of age .At 60 days of age ,eight of mature female Wistar rats from each group were anesthetized, dissected and blood samples were obtained from abdominal vein for assessment of Estrogen concentration in serum.

Ovaries and uteri were also obtained, weighted and fixed in formalin 10% for histopathological study.Ovarian and uterine weights of treated group recorded significant decrement in comparison with that of control.Hormonal assay in sera showed significant decrement of estrogen in treated mature female rats in comparison with that of control female rats.

Ovarian sections of treated female rats revealed lower level of proliferation in the follicular tissue and predominance of Graffian follicles compared with control.

Similarly uterine sections of treated female rats showed filtration of adipose tissue with less proliferated changes and less uterine glands compared with that in control.