Extraction and purification of Salmonella spp. enterotoxin isolated from bovine in Basrah province

Abstract EN

One Hundred eighty fecal samples and (50) bile samples were collected from cattle of different ages and both sexes present in Basrah farms and Slaughterhouse.

The results of the bacteriological and serological methods carried out on fecal and bile samples of cows detect Salmonella spp in the fecal samples of 3 cows (1.66% ) and these bacteria were not detected (0%) in bile samples.

Concerning the effect of months of study on the rate of Salmonella spp.

isolation .

The higher rate of isolation was encountered in march (6.66%) followed by February (2.38%), while in other months no Salmonella isolates were observed.

Depending on the sex of animals the higher rate of Salmonella isolation was observed in males %)2.06) and it was in females (1.204%) .

According to age group the higher rate of Salmonella isolation (%5.9)was observed in the third age group ) followed by the second age group (1<-3) in which the rate was ( %2.09) .

There was statistical significance difference (p< 0.05) among age groups concerning the Salmonella isolation rate.

Suckling mice and permeability of rabbit skin were show good result for detection of enterotoxin which were extracted from the more virulent isolate No.

(161).

The enterotoxin then were purified and fractionated by gel flirtation on sephadex (G-100).

Results of gel flirtation showed that the toxin had two peaks , one of them were highly toxic.

The chemical studying of enterotoxin characteristics revealed that it contained sugar moiety and it was a glycoprotein.