Study of some serum biochemical changes of experimental diabetes mellitus in donkey

Abstract EN

This study was conducted to induce experimental diabetes in donkeys and assess some serum biochemical changes in diabetes mellitus.Fifteen donkeys were used in this experiment and divided into two groups: A.

Treatment group which include (11) donkeys .

Diabetic group.

denoted by Dm.

B.

Control group which includes (4) donkeys denoted by Cm.

Animals were housed in field .The animals were left tow weeks for adaptation.

Diabetes mellitus was induced by i.v injection of alloxan monohydrate at dose rate 100 mg/kg dissolved in 25 ml of normal saline, while the control groups were injected with 25 ml of normal saline.

Blood was collected before injection of alloxan monohydrate and after that (10,20.30,40)days to check fasting and random serum glucose.The laboratory tests including serum glucose, serum urea, serum creatinine, serum cholesterol, serum lactate dehydrogenase , We found out the following result according to the periods of the experiments: Serum glucose level was elevated starting from the 1st 10 days period after induction of diabetes mellitus.

It reached its peak levels on day(20,30,40).

Serum urea, serum creatinine, serum cholesterol, serum lactate dehydrogenase were elevated in diabetic donkeys,in control groups, but there were no significant difference.