Detection of Salmonella typhimurium in chicken meat imported in the local markets of Diwaniya city by using PCR technique

Abstract EN

The aim of this study to detected contamination with Salmonella spp.

In imported chicken meat in the local markets of Al- Diwaniyia city .

to protect health of consumer and Determintion the most contaminated origin with salmonella spp.

A toatl of 100 chicken meat samples collected from different origin.

The bacteria cultured and isolated in enrichment and selective media .

Salmonella isolates were subjected to some biochemical tests show positive productive results H2S .TSI .

SIM And its give negative for indole , vo-gs Proskauer and ureas , Biochemical identification was carried out using API 20-E test ..the result showed isolation sample (3355)60% on bismuth sulphate agar and the results of isolation on chromogenic agar were 87.8 |%(2933) .according to reading Api20-E system the results of confirmation of isolates 92%(2526) In this study,( 23) Salmonella isolates were detected by polymerase chain reaction (PCR) by using 16s rRNA and invA gene these primers were selected specifically for the detection of Salmonella to amplify a 406bp and 558 bp DNA fragments, respectively.

Only 7 isolates out of 23 were identified as S.

typhimurium the results of this study showed the highest percent of s.typhimurim isolates was ( 50%) ( 3/6) for India origin and the lowest was Turkish origin