استخدام تقنية ال (PCR) Real time للكشف الجزيئي لجينات hlyA و vfhly في بكتيريا Vibrio cholera و Vibrio fluvialis المعزولة من الأسماك
Other Title(s)
Used PCR technology (Real Time) for discovering about the responsible genetic to gen hly and vfhly for isolation and diagnosis Vibrio cholerae and Vibrio fluvialis bacteria from fish
Joint Authors
التميمي، منال بادي صالح
الجبوري، عذراء عودة حسين
الحسيني، خديجة صادق جعفر
Source
مجلة جامعة ذي قار للبحوث الزراعية
Issue
Vol. 4, Issue 2 (31 Dec. 2015), pp.182-201, 20 p.
Publisher
University of Thi-Qar College of Agricultural and Marshes
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
20
Main Subjects
Topics
- Fishes
- Physiological effects
- Twenty-first century
- Nasiriyah
- Polymerase chain reaction
- Diagnosis
- Molecular aspects
Abstract EN
In this study, there was isolating and identifying Vibrios bacteria that taken from multi-kinds of fresh and frozen fish,such as Suoboor Tenualosa ilisha , Silver carp Hypophthalmichthys molitrix, Beni Barbus sharpey and Shanak Spondyliosoma cantharus, we collected 210 fish from Basrah market (Big market in Basrah, Al-Ashar, Al- Tanoma and Khmsa meel) while we collected 150 fish from Silver carp, Beni and Shanak from Nasiriyah market (Big market and Harj market)this collecting process of samples taken a period from 2-June to 30- November \2013.
These fish bring to laboratory and the samples takenfrom it and planted directly on agricultural medians.
(nutrient agar, blood agar, MacConkey agar and selective media (Thiosulphate Citrate Bile salt Sucrose Agar TCBS) then the dishes were incubated aerobically at temperature 37cº for 18 – 24 hours.
After the period of incubation, we were identifying 30 positive samples from Vibrios and this genus relianced to the shap of cultures and cell under microscope and by Catalase test ,Oxidase test and motion test afer that identifying the types relianced to biochemical tests and using certain API20E system, that indenting two kinds from Vibrios bacteria Vibrio cholera and Vibrio fluvialis.
we confirmed that fresh fish including these bacteria more than frozen fish Results clarified When we used PCR technology (Real Time) for discovering about the responsible genetic from hemolysim enzyme in Vibrio spp., that 27 samples from Vibrio cholera bacteria and 3 samples from Vibrio fluvialis bacteria have this Enzyme who analyzed red blood cell (RBC) which considered from Tocsins that belong to multi Vibrio spp.
that assist to sick.
American Psychological Association (APA)
الحسيني، خديجة صادق جعفر والجبوري، عذراء عودة حسين والتميمي، منال بادي صالح. 2015. استخدام تقنية ال (PCR) Real time للكشف الجزيئي لجينات hlyA و vfhly في بكتيريا Vibrio cholera و Vibrio fluvialis المعزولة من الأسماك. مجلة جامعة ذي قار للبحوث الزراعية،مج. 4، ع. 2، ص ص. 182-201.
https://search.emarefa.net/detail/BIM-755471
Modern Language Association (MLA)
الحسيني، خديجة صادق جعفر....[و آخرون]. استخدام تقنية ال (PCR) Real time للكشف الجزيئي لجينات hlyA و vfhly في بكتيريا Vibrio cholera و Vibrio fluvialis المعزولة من الأسماك. مجلة جامعة ذي قار للبحوث الزراعية مج. 4، ع. 2 (2015)، ص ص. 182-201.
https://search.emarefa.net/detail/BIM-755471
American Medical Association (AMA)
الحسيني، خديجة صادق جعفر والجبوري، عذراء عودة حسين والتميمي، منال بادي صالح. استخدام تقنية ال (PCR) Real time للكشف الجزيئي لجينات hlyA و vfhly في بكتيريا Vibrio cholera و Vibrio fluvialis المعزولة من الأسماك. مجلة جامعة ذي قار للبحوث الزراعية. 2015. مج. 4، ع. 2، ص ص. 182-201.
https://search.emarefa.net/detail/BIM-755471
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 197-200
Record ID
BIM-755471