استخدام تقنية ال (PCR)‎ Real time للكشف الجزيئي لجينات hlyA و vfhly في بكتيريا Vibrio cholera و Vibrio fluvialis المعزولة من الأسماك

Abstract EN

In this study, there was isolating and identifying Vibrios bacteria that taken from multi-kinds of fresh and frozen fish,such as Suoboor Tenualosa ilisha , Silver carp Hypophthalmichthys molitrix, Beni Barbus sharpey and Shanak Spondyliosoma cantharus, we collected 210 fish from Basrah market (Big market in Basrah, Al-Ashar, Al- Tanoma and Khmsa meel) while we collected 150 fish from Silver carp, Beni and Shanak from Nasiriyah market (Big market and Harj market)this collecting process of samples taken a period from 2-June to 30- November \2013.

These fish bring to laboratory and the samples takenfrom it and planted directly on agricultural medians.

(nutrient agar, blood agar, MacConkey agar and selective media (Thiosulphate Citrate Bile salt Sucrose Agar TCBS) then the dishes were incubated aerobically at temperature 37cº for 18 – 24 hours.

After the period of incubation, we were identifying 30 positive samples from Vibrios and this genus relianced to the shap of cultures and cell under microscope and by Catalase test ,Oxidase test and motion test afer that identifying the types relianced to biochemical tests and using certain API20E system, that indenting two kinds from Vibrios bacteria Vibrio cholera and Vibrio fluvialis.

we confirmed that fresh fish including these bacteria more than frozen fish Results clarified When we used PCR technology (Real Time) for discovering about the responsible genetic from hemolysim enzyme in Vibrio spp., that 27 samples from Vibrio cholera bacteria and 3 samples from Vibrio fluvialis bacteria have this Enzyme who analyzed red blood cell (RBC) which considered from Tocsins that belong to multi Vibrio spp.

that assist to sick.