Micropropagation of Crotone (Codiaeum variegatum)‎

Abstract EN

The present study was conducted to investigate the effects of different concentration and combinations of plant growth regulators on callus induction and plant regeneration of croton (Codiaeum variegatum).

The leaf segments were used as explant and cultured on MS medium supplemented with different concentrations of NAA or 2,4-D combined with BA for callus induction.

The best callus was formed on whole area of explant cultured on MS medium with different levels of BA combined with different concentrations of 2,4-D.

MS medium supplemented with 2mgl-1 BA+ 0.2 mgl-1 2,4-D was used for percentage of callus induction (100%) and the highest average callus fresh weight (5.39 mg) was formed under dark condition.

The highest percentage of callus induction (80%) and the highest average callus fresh weight (3.91 mg) was obtained under light condition compared with control treatment which did not formed callus.

In multiplication stage the the maximum number of shoots, number of leaves / shoot and highest length of shoots (4.58 shoots/culture, 3.13 leaves / shoot and 2.33 cm) was obtained in MS medium supplemented with 2.5mgl-1 BA respectively, compared with all treatments including the treatment control.

For shoot multiplication, the maximum (4.53) shoot/ culture were formed on MS medium having 3mgl-1 BA+ 4.5 mgl-1 Kin.

At rooting stage, a large number of roots, root length and root percentage (2.67 roots /explant, 3.56 cm and 70%) respectively, was observed when 0.5 mgl-1 IBA was used in ¼ MS medium