Micropropagation of Crotone (Codiaeum variegatum)
Other Title(s)
الإكثار الدقيق لنبات الكروتون Codiaeum variegatum
Joint Authors
Duhoky, Muslih M. S.
al-Mizuri, Layla S. M.
Source
Issue
Vol. 13, Issue 1 العلوم الزراعية و البيطرية (30 Jun. 2010), pp.112-121, 10 p.
Publisher
Publication Date
2010-06-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
The present study was conducted to investigate the effects of different concentration and combinations of plant growth regulators on callus induction and plant regeneration of croton (Codiaeum variegatum).
The leaf segments were used as explant and cultured on MS medium supplemented with different concentrations of NAA or 2,4-D combined with BA for callus induction.
The best callus was formed on whole area of explant cultured on MS medium with different levels of BA combined with different concentrations of 2,4-D.
MS medium supplemented with 2mgl-1 BA+ 0.2 mgl-1 2,4-D was used for percentage of callus induction (100%) and the highest average callus fresh weight (5.39 mg) was formed under dark condition.
The highest percentage of callus induction (80%) and the highest average callus fresh weight (3.91 mg) was obtained under light condition compared with control treatment which did not formed callus.
In multiplication stage the the maximum number of shoots, number of leaves / shoot and highest length of shoots (4.58 shoots/culture, 3.13 leaves / shoot and 2.33 cm) was obtained in MS medium supplemented with 2.5mgl-1 BA respectively, compared with all treatments including the treatment control.
For shoot multiplication, the maximum (4.53) shoot/ culture were formed on MS medium having 3mgl-1 BA+ 4.5 mgl-1 Kin.
At rooting stage, a large number of roots, root length and root percentage (2.67 roots /explant, 3.56 cm and 70%) respectively, was observed when 0.5 mgl-1 IBA was used in ¼ MS medium
American Psychological Association (APA)
Duhoky, Muslih M. S.& al-Mizuri, Layla S. M.. 2010. Micropropagation of Crotone (Codiaeum variegatum). Journal of Dohuk University،Vol. 13, no. 1 العلوم الزراعية و البيطرية, pp.112-121.
https://search.emarefa.net/detail/BIM-756266
Modern Language Association (MLA)
Duhoky, Muslih M. S.& al-Mizuri, Layla S. M.. Micropropagation of Crotone (Codiaeum variegatum). Journal of Dohuk University Vol. 13, no. 1 Agricultural and Veterinary Sciences (2010), pp.112-121.
https://search.emarefa.net/detail/BIM-756266
American Medical Association (AMA)
Duhoky, Muslih M. S.& al-Mizuri, Layla S. M.. Micropropagation of Crotone (Codiaeum variegatum). Journal of Dohuk University. 2010. Vol. 13, no. 1 العلوم الزراعية و البيطرية, pp.112-121.
https://search.emarefa.net/detail/BIM-756266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 118-120
Record ID
BIM-756266