Regeneration of F1 hybrids of eggplant (Solanum melongena L.)‎ and tomato (Lycopersicon esculentum L.)‎ from stem explants

Abstract EN

An in vitro method for propagation of eggplant (Solanum melongena L.) and tomato (Lycopersicon esculentum L.) was developed.

Stem explants of Fl hybrids of eggplant and tomato from in vitro germinated seedlings were cultured on MS medium supplemented with different concentrations and combinations of BA and NAA.

Multiples shoots were induced from the stem explants of eggplant and tomato by culturing them on MS medium supplemented with 2.5, 5.0 mgl-1 BA plus 0.3 mgl-1 IAA or 2.5mgl-1 BA plus 0.2 mgl-1 NAA.

Differentiation and shoots were initiated after one week of culture and after 8 weeks of primary culture, an average of three plantlets were developed from the cultures supplemented with 5.0 mgl-1 BA in eggplant and 2.5 mgl-1 BA in tomato.

The shoots when subcultured on 1/2 salt strength MS medium supplemented with 0.3 mgl-1 NAA produced plantlets subculture with well-developed root system after 4 weeks.

The plantlets were then acclimatized, and planted in pots which showed 95% survival under natural conditions