Ovarian response in super ovulated karadi yerling ewes treated with insulin

Abstract EN

Twenty five Karadi yearling ewes and 52 kg in weight were divided randomly into two groups;Group one (control, n= 13) and Group two (insulin treatment, n=12).

Estrus was induced first by intravaginal sponges and PMSG .

Following estrus were pre-treated with HCG and estradiol and animals in both groups were superovulated using 20 IU FSH i.m.

in six divided descending doses .i.e.

4/4 and 3/3 and 3/3 at 12 h interval for three consecutive days .Prior to gonadotropins adminstarion, Animals in group one were given a normal saline and group two animals were given long acting purified bovine insulin 0.2 IU /kg body weight per day s.c.

for three consecutive days started on day 7 of the estrus cycle.

After PGF2α treated, two rams fitted with marking crayons were introduced with ewes for detection of estrus, and then bred with natural service at approximately 12 hrs interval until the end of estrus.

Three days following mating, all animals were sacrificed to determine ovulation rate.

Results revealed that percent ewes responded to treatment were 69.23% for control group and 75.00% for insulin treated group.

Ovulation rate averaged 1.44±0.22 and 1.56±0.24 for the control and insulin groups, respectively, and the unovulated large follicles /animal averaged 0.50±0.19 and 0.84±0.19, for control and insulin group, respectively.Percent ova recovered was 84.6 and 35.7% for control and treated groups, respectively.