عزل و تشخيص بكتريا Enterobacteriaceae و تحديد الأنواع المنتجة للهستامين في أسماك الضلعة Scomberoides commersonianus و الخباط Chirocentrus dorab و الحمام Megalaspis cordyla و الصبور Tenualosa ilisha

Abstract EN

Geniuses and species of Enterobacteriaceae was isolated and identification from various of fish Scomberoides commersonianus , Chirocentrus dorab , Megalaspis cordyla and Tenualosa ilisha , which have been brought from region of FAO in the province of Basrah, All samples were examined for the occurrence of bacteria and were identified according to growth characteristics , morphological, and microscopic examinations in addition to biochemical tests and API Hi 25 TM Enterobacteriaceae Identification Kit .

Sixteen isolates of bacteria were found and as a follow : Klebsiella pneumonia sub.

pneumonia, Klebsiella oxytoca, Morganella morganii sub.

Morganii, Enterobacter aerogenes, Enterobacter cloacae , Enterobacter sakazakii , Enterobacter taylorae, Citrobacter freundii , Citrobacter amalonaticus , Proteus marina, Proteus vulgaris , Proteus mirabilis, Hafnia alvei , Escherichia coli, Serratia marcescens and Serratia fonticola .

The results of determination of histamine which produced from bacteria by using solid and liquid media showed that Morganella morganii sub.

Morganii, Enterobacter aerogenes , Klebsiella oxytoca, Enterobacter cloacae, Entrobacter sakazakii , Entrobacter taylorae, Proteus marina, Proteus mirabilis, Proteus vulgaris , Citrobacter freundii and Citrobacter amalonaticus were produce high level of histamine , while Escherichia coli ,Serratia marcescens and Serratia fonticola were produce low level of histamine .

On the other hand , Hafnia alvei was not produce histamine .