Investigation of the chemical composition and different effects of a rumex dentatus methanol extract against drug resistant pseudomonas aeruginosa isolates

Abstract EN

Background: The emergence of Pseudomonas aeruginosa as a multiple drug resistant bacteria has renewed efforts to identify alternative antibacterial materials, such as medicinal plants.

Objectives: The aims of this study were to investigate the antibacterial effects of a Rumex dentatus methanol extract against drug resistant P.

aeruginosa isolates and to analyze the chemical composition of this extract.

Materials and Methods: Sixty strains of P.

aeruginosa were isolated from urine and wound samples and identified using conventional tests, as well as 16S rDNA gene sequencing.

Results: The isolates were completely resistant to the tested antibiotics, with minimum inhibitory concentration (MIC) values for ceftazidime, imipenem, and gentamicin between 256 to > 256 g/mL, 8 to > 256 g/mL, and 64 to > 256 g/mL, respectively.

The R.

dentatus extract had a broad spectrum activity against all tested isolates and maximum growth inhibition against isolates from burn wound and urinary tract infections occurred at 200 mg/mL (22.43mm and 22.86mm, respectively).

The means of the MIC and minimum bactericidal concentration (MBC) values were 25 and 50 mg/mL, respectively.

The effects of a combination of the methanol extract and ceftazidime, imipenem, and gentamicin antibiotics were antagonistic.

The GC-MS analysis of the methanol extract revealed a high probable presence percentage of alpha-pinene, sabinene, and eucalyptol (1, 8-cineole) that have confirmed antimicrobial activity.

Conclusions: The R.

dentatus methanol extract with broad spectrum activity can be viewed as an alternative to antibiotics for treatment of P.

aeruginosa infection.