Molecular diagnosis of Chlamydia trachomatis in infertile Iraqi women using Real time-PCR and comparison with other methods
Other Title(s)
التشخيص الجزيئي لل Chlamydia trachomatis في النساء العراقيات العقيمات باستخدام Real time-PCR و المقارنة مع طرق أخرى
Joint Authors
al-Awwadi, Salwa Jabir Abd Allah
Sad al-Din, Shuruq Muhammad Kazim
Muhammad, Iqbal Harbi
Source
Issue
Vol. 58, Issue 3B (30 Sep. 2017), pp.1427-1436, 10 p.
Publisher
University of Baghdad College of Science
Publication Date
2017-09-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
- Woman
- Sexually transmitted diseases
- Infertility
- Iraq
- Polymerase chain reaction
- Diagnosis
- Molecular aspects
Abstract EN
Chlamydia trachomatis is the most common of negative gram bacteria that cause sexually transmitted diseases.
It affects the reproductive system in women, not the symptoms of the disease, but the most serious is the long-term effects of the reproductive system..
out of 100 women were attending different hospitals in Baghdad included the Gynaecology Departments of Women Health Center at Al-Elwyia Obstetrics Hospital .
Ibn Al balady Maternity and Children's Hospital , Kamal al-Samarrai hospital Fertility Center infertility treatment and In Vitro Fertilization ( IVF ) (20 control and 80 women with infertility) DNA was extracted from the Endocervical Swabs of all infertili women, to investigate the bacteria by using Real time -PCR technique and another swab were subjected to direct Rapid Test .
The venous blood sample was obtained, serum were diagnosis by Anti- Chlamydia trachomitis Chlamydia trachomitis IgG ELISA.
The result of the Real time-PCR revealed was significant associated with infertility women (OR= 0.733; 13.75%, P= 0.041), among 80 infertili women, while was no significant diagnosis by ELISA (OR= 0.063; 2.50%, p= 0.095).
and no significant associated with Rapid Test .
In summary Real time-PCR has the possibility to contribute in the primary screening for infertility in women
American Psychological Association (APA)
Muhammad, Iqbal Harbi& al-Awwadi, Salwa Jabir Abd Allah& Sad al-Din, Shuruq Muhammad Kazim. 2017. Molecular diagnosis of Chlamydia trachomatis in infertile Iraqi women using Real time-PCR and comparison with other methods. Iraqi Journal of Science،Vol. 58, no. 3B, pp.1427-1436.
https://search.emarefa.net/detail/BIM-758626
Modern Language Association (MLA)
Muhammad, Iqbal Harbi…[et al.]. Molecular diagnosis of Chlamydia trachomatis in infertile Iraqi women using Real time-PCR and comparison with other methods. Iraqi Journal of Science Vol. 58, no. 3B (2017), pp.1427-1436.
https://search.emarefa.net/detail/BIM-758626
American Medical Association (AMA)
Muhammad, Iqbal Harbi& al-Awwadi, Salwa Jabir Abd Allah& Sad al-Din, Shuruq Muhammad Kazim. Molecular diagnosis of Chlamydia trachomatis in infertile Iraqi women using Real time-PCR and comparison with other methods. Iraqi Journal of Science. 2017. Vol. 58, no. 3B, pp.1427-1436.
https://search.emarefa.net/detail/BIM-758626
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-758626