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A method for purification of plant viruses belonging to different virus groups
Other Title(s)
طريقة لتنقية فيروسات نباتية تنتمي إلى مجموعات فيروسية مختلفة
Author
Source
Assiut University Bulletin for Environmental Researches
Issue
Vol. 18, Issue 2 (31 Oct. 2015), pp.1-7, 7 p.
Publisher
Assiut University Center for Environmental Studies and researches
Publication Date
2015-10-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A method for isolation and purification of plant viruses was described.
The method involved use of organic solvents, differential centrifugation and density gradient centrifugation on step and linear caesium sulphate gradients with which a sucrose cushion was used.
The method was found suitable for purification of a range of plant viruses belonging to different virus groups and representing the different shapes reported for plant viruses.
The plant viruses purified through this method included Potato virus Y (PVY), Tobacco etch virus (TEV), Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tomato yellow leaf curl virus (TYLCV).
Both the step and linear gradients of caesium sulphate gave similar results in isolation and purification of these viruses and no appreciable differences were observed between them when either of which was used
American Psychological Association (APA)
Abd Allah, Umar Ahmad. 2015. A method for purification of plant viruses belonging to different virus groups. Assiut University Bulletin for Environmental Researches،Vol. 18, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-759617
Modern Language Association (MLA)
Abd Allah, Umar Ahmad. A method for purification of plant viruses belonging to different virus groups. Assiut University Bulletin for Environmental Researches Vol. 18, no. 2 (Oct. 2015), pp.1-7.
https://search.emarefa.net/detail/BIM-759617
American Medical Association (AMA)
Abd Allah, Umar Ahmad. A method for purification of plant viruses belonging to different virus groups. Assiut University Bulletin for Environmental Researches. 2015. Vol. 18, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-759617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6
Record ID
BIM-759617