Effect of tamoxifen citrate supplement to SMART medium on human sperm DNA fragmentation during in vitro sperm activation

Abstract EN

In vitro sperm activation by adding some materials was found to be very important methods to deals with DNA fragmentation of sperms which was one of causes of male infertility.

The objective of the present study was to investigate the effect of different concentrations of tamoxifen citrate (TC) on the outcome of in vitro human sperm activation.

One hundred semen samples of infertile and fertile men from High Institute of Infertility Diagnosis and ART, Al-Nahrain University with range of age (32.21 ± 0.83) years and their mean duration of infertility (5.26 ± 0.41) years were used.

Before using centrifugation swim-up technique, the washed samples were divided into 3 groups : control group (G1) without TC, while in G2 and G3, two concentrations of TC (20μg / mL and 40μg / mL) were added respectively.

The three groups were enriched with SMART medium.

Then, sperm DNA fragmentation was evaluated after the addition of TC.

Data were statistically analyzed using complete randomized design (CRD).

The results of DNA fragmentation in post-activation revealed a significant decreased (P≤0.05) in treated groups (G2 and G3), compared with pre-activation and control group (G1).

However, a significant (P<0.05) decreased was appeared in high concentration of TC when compared with other treated group.

Based on the results of this study, it can be concluded that the addition of 40μg / mL of TC to washed sperms can improve sperm DNA integrity in vitro.-