Effect of tamoxifen citrate supplement to SMART medium on human sperm DNA fragmentation during in vitro sperm activation
Other Title(s)
تأثير تاموكسفين سترات المضاف إلى الوسط الزرعي SMART على تشظي دنا النطف البشرية خلال تنشيط النطف خارج الجسم
Joint Authors
Yinzil, Jabbar Hamid
Kazim, Nur Karim
Fakhr al-Din, Muhammad Baqir Muhammad Rashad
Source
Issue
Vol. 57, Issue (sA) (31 Dec. 2016), pp.20-25, 6 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
In vitro sperm activation by adding some materials was found to be very important methods to deals with DNA fragmentation of sperms which was one of causes of male infertility.
The objective of the present study was to investigate the effect of different concentrations of tamoxifen citrate (TC) on the outcome of in vitro human sperm activation.
One hundred semen samples of infertile and fertile men from High Institute of Infertility Diagnosis and ART, Al-Nahrain University with range of age (32.21 ± 0.83) years and their mean duration of infertility (5.26 ± 0.41) years were used.
Before using centrifugation swim-up technique, the washed samples were divided into 3 groups : control group (G1) without TC, while in G2 and G3, two concentrations of TC (20μg / mL and 40μg / mL) were added respectively.
The three groups were enriched with SMART medium.
Then, sperm DNA fragmentation was evaluated after the addition of TC.
Data were statistically analyzed using complete randomized design (CRD).
The results of DNA fragmentation in post-activation revealed a significant decreased (P≤0.05) in treated groups (G2 and G3), compared with pre-activation and control group (G1).
However, a significant (P<0.05) decreased was appeared in high concentration of TC when compared with other treated group.
Based on the results of this study, it can be concluded that the addition of 40μg / mL of TC to washed sperms can improve sperm DNA integrity in vitro.-
American Psychological Association (APA)
Kazim, Nur Karim& Yinzil, Jabbar Hamid& Fakhr al-Din, Muhammad Baqir Muhammad Rashad. 2016. Effect of tamoxifen citrate supplement to SMART medium on human sperm DNA fragmentation during in vitro sperm activation. Iraqi Journal of Science،Vol. 57, no. (sA), pp.20-25.
https://search.emarefa.net/detail/BIM-759659
Modern Language Association (MLA)
Kazim, Nur Karim…[et al.]. Effect of tamoxifen citrate supplement to SMART medium on human sperm DNA fragmentation during in vitro sperm activation. Iraqi Journal of Science Vol. 57, no. (sA) (2016), pp.20-25.
https://search.emarefa.net/detail/BIM-759659
American Medical Association (AMA)
Kazim, Nur Karim& Yinzil, Jabbar Hamid& Fakhr al-Din, Muhammad Baqir Muhammad Rashad. Effect of tamoxifen citrate supplement to SMART medium on human sperm DNA fragmentation during in vitro sperm activation. Iraqi Journal of Science. 2016. Vol. 57, no. (sA), pp.20-25.
https://search.emarefa.net/detail/BIM-759659
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 23-25
Record ID
BIM-759659