Measurement and purification of alanine aminotransferase (ALT) enzyme activity in patients with celiac disease
Other Title(s)
قياس و تنقية فعالية أنزيم الأنين امينوترانسفيريز (ALT) في المرضى المصابين بحساسية الحنطة
Joint Authors
Murtada, Janan Husayn
Abd al-Razzaq, Iman Hashim
Muhammad, Taghrid Ulum
Source
Issue
Vol. 14, Issue 3 (30 Sep. 2017), pp.557-563, 7 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2017-09-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract EN
Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance.
The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease.
Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year.
In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p<0.01).
The ALT enzyme was partial purified from sera women with celiac disease by dialysis, gel filtration using Sephadex G- 50 and ion exchange chromatography using DEAE- cellulose A-50 .
The results showed a single peak by using gel filtration and the activity reached 31-15 U/L .Two isoenzymes were obtained by using ion exchange chromatography and the purity degree of isoenzymse (I, II) were (5.7) and (5.53) fold respectively-
American Psychological Association (APA)
Muhammad, Taghrid Ulum& Murtada, Janan Husayn& Abd al-Razzaq, Iman Hashim. 2017. Measurement and purification of alanine aminotransferase (ALT) enzyme activity in patients with celiac disease. Baghdad Science Journal،Vol. 14, no. 3, pp.557-563.
https://search.emarefa.net/detail/BIM-759922
Modern Language Association (MLA)
Muhammad, Taghrid Ulum…[et al.]. Measurement and purification of alanine aminotransferase (ALT) enzyme activity in patients with celiac disease. Baghdad Science Journal Vol. 14, no. 3 (2017), pp.557-563.
https://search.emarefa.net/detail/BIM-759922
American Medical Association (AMA)
Muhammad, Taghrid Ulum& Murtada, Janan Husayn& Abd al-Razzaq, Iman Hashim. Measurement and purification of alanine aminotransferase (ALT) enzyme activity in patients with celiac disease. Baghdad Science Journal. 2017. Vol. 14, no. 3, pp.557-563.
https://search.emarefa.net/detail/BIM-759922
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 561-562
Record ID
BIM-759922