Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease

Abstract EN

Coronary artery disease (CAD) is the most frequent cause of morbidity and mortality in the world and it is known as a multifactorial disorder which is influenced by both genetic and environmental factors.

Based on different assays, the platelet derived growth factor B (PDGF-B) gene is shown to be amongst the inflammation inducers involved in different pathological conditions such as atherosclerosis.

Aim : In this case-control study we have examined the association of the functional PDGF-B +286 and +1135 polymorphisms and its expression status with susceptibility to CAD.

Subjects and methods: Study groups included 369 patients with CAD and 413 healthy individuals.

Genotypic and allelic frequencies of PDGF-B +286 and +1135 polymorphisms were determined by the SSP-PCR method.

PDGF-B expression status was identified by quantitative real-time PCR.

Results : The analysis of genotyping results revealed CAD patients had an increased frequency of PDGF-B +286A>G A/A genotype in comparison with healthy individuals.

Furthermore, we found that the PDGF-B expression level in CAD patients group is nearly 2-folds greater than its level in control group.

Conclusion: There is probably a relationship between variations in PDGF-B gene and CAD influence.

The increase in PDGF-B gene expression may has a role in susceptibility to CAD.