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Extremely low frequency electromagnetic field in combination with B-lapachone up-regulates the genes of non-homologous end joining
Joint Authors
Sanie Jahromi, Fatimah
Mahmoudinasab, Hamidah
Sadat, Mustafa
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 18, Issue 4 (31 Oct. 2017), pp.389-392, 4 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2017-10-31
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Background and purpose : Non-homologous end joining (NHEJ) is the major pathway for removing DNA double strand breaks lesions.
NHEJ is considered to be a resistant factor against chemotherapy induced neuropathy.
b-Lapachone (b-Lap) is one of the antineoplastic agents which is shown to have anti neuroinflammatory effects.
Extremely low frequency (<300 Hz) electromagnetic field (EMF) is shown to decrease NHEJ genes expression.
Morphine (Mor) is associated with reducing effect on DNA repair and induce DNA damages.
The goal of this study was to evaluate the effect of combination treatment of b-Lap, morphine (Mor) and EMF on expression of NHEJ related genes (XRCC4, Ku70, Ku80, DNA-PKcs and LIG4).
Materials and methods: SH-SY5Y cells (epithelial neuroblasts) were treated with four combinational treatments of b-Lap (2.0 and 3.2 lM), Mor (5.0 lM) and EMF (50 Hz, 0.50 mT, ‘‘15 min field on/15 min field off”) and mRNA levels of XRCC4, Ku70, Ku80, DNA-PKcs and LIG4 were evaluated by quantitative realtime PCR and primers specific for the examined genes.
The experiments were done in triplicates.
Results : No significant alteration in the mRNA levels of NHEJ related genes was observed in ‘‘b-Lap alone” and ‘‘b-Lap + Mor” treated cells.
The expression levels of NHEJ related genes were significantly increased in ‘‘b-Lap + EMF” and ‘‘b-Lap + Mor + EMF”.
Multiple linear regression analysis showed that the effect of EMF and Mor on NHEJ related genes expression is opposite to the effect of b-Lap.
Conclusion: In overall, combination of b-Lap, Mor and EMF leads to increased expression of NHEJ related gene expression.
This effect may lead to decreased sensitivity of SH-SY5Y cells against b-Lap and can improve its neuroprotective property which might be hopeful for its clinical applications.
American Psychological Association (APA)
Sanie Jahromi, Fatimah& Mahmoudinasab, Hamidah& Sadat, Mustafa. 2017. Extremely low frequency electromagnetic field in combination with B-lapachone up-regulates the genes of non-homologous end joining. The Egyptian Journal of Medical Human Genetics،Vol. 18, no. 4, pp.389-392.
https://search.emarefa.net/detail/BIM-760315
Modern Language Association (MLA)
Sanie Jahromi, Fatimah…[et al.]. Extremely low frequency electromagnetic field in combination with B-lapachone up-regulates the genes of non-homologous end joining. The Egyptian Journal of Medical Human Genetics Vol. 18, no. 4 (Oct. 2017), pp.389-392.
https://search.emarefa.net/detail/BIM-760315
American Medical Association (AMA)
Sanie Jahromi, Fatimah& Mahmoudinasab, Hamidah& Sadat, Mustafa. Extremely low frequency electromagnetic field in combination with B-lapachone up-regulates the genes of non-homologous end joining. The Egyptian Journal of Medical Human Genetics. 2017. Vol. 18, no. 4, pp.389-392.
https://search.emarefa.net/detail/BIM-760315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 392
Record ID
BIM-760315