Circulating Mirna-21 and programed cell death (PDCD) 4 gene expression in hepatocellular carcinoma (HCC) in Egyptian patients
Joint Authors
Ghanayim, Najla Muhammad
al-Jiddawi, Jamal
Ubada, Manar
al-Sayyid, Halah Hasan
Kilani, Ayman
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 18, Issue 2 (30 Apr. 2017), pp.137-145, 9 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2017-04-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background: Circulating microRNAs (miRNAs) are endogenous, small (17–25 nucleotides) non-coding RNAs that are overexpressed in many human cancers including hepatocellular carcinoma (HCC).
Moreover, circulating miRNAs can reflect the level of tissue miRNAs, so could be potential tumor markers.
miRNA-21 regulates post-transcriptional expression of tumor suppressor gene; programed cell death 4 (PDCD4) gene which implies that miRNA-21 might be a novel diagnostic and/or prognostic marker for cancer.
Objective: To evaluate the diagnostic and prognostic potential of circulating miRNA-21 and study the expression of PDCD4 gene as a target of miRNA-21 in HCC in Egyptian patients.
Subjects and methods: This study was conducted on 30 HCC patients, 20 chronic liver disease (CLD) patients due to HCV infection and 20 healthy subjects.
Serum alpha fetoprotein (AFP) was measured for all participants.
The relative plasma expression of each of miRNA-21 and PDCD4 gene was determined in whole blood samples using real-time polymerase chain reaction.
Results: The results revealed over expression of miRNA-21 and under expression of PDCD4 gene in HCC group (p< 0.05) compared to both CLD and healthy subjects, while no significant change was detected between CLD and healthy subjects.
miRNA-21 expression was negatively correlated with PDCD4 gene expression.
miRNA-21 expression increased significantly with presence of cirrhosis, increased number of focal lesions, larger size of tumor, advanced tumor stage and presence of vascular invasion.
Receiver Operator of Characteristics (ROC) curve analysis of plasma miRNA-21 revealed that, at a cut-off value of 3.93 (fold expression), the sensitivity and specificity for differentiation of HCC cases were 93% and 90%, respectively.
Conclusion: Circulating miRNA-21 could be a novel early diagnostic and prognostic biomarker for detection of HCC.
Approaches interfering with the miRNA-21/PDCD4-axis, or releasing PDCD4 expression, may have a strong basis for therapeutic uses in cancer in the future.
American Psychological Association (APA)
al-Jiddawi, Jamal& Ubada, Manar& Kilani, Ayman& al-Sayyid, Halah Hasan& Ghanayim, Najla Muhammad. 2017. Circulating Mirna-21 and programed cell death (PDCD) 4 gene expression in hepatocellular carcinoma (HCC) in Egyptian patients. The Egyptian Journal of Medical Human Genetics،Vol. 18, no. 2, pp.137-145.
https://search.emarefa.net/detail/BIM-760885
Modern Language Association (MLA)
al-Jiddawi, Jamal…[et al.]. Circulating Mirna-21 and programed cell death (PDCD) 4 gene expression in hepatocellular carcinoma (HCC) in Egyptian patients. The Egyptian Journal of Medical Human Genetics Vol. 18, no. 2 (Apr. 2017), pp.137-145.
https://search.emarefa.net/detail/BIM-760885
American Medical Association (AMA)
al-Jiddawi, Jamal& Ubada, Manar& Kilani, Ayman& al-Sayyid, Halah Hasan& Ghanayim, Najla Muhammad. Circulating Mirna-21 and programed cell death (PDCD) 4 gene expression in hepatocellular carcinoma (HCC) in Egyptian patients. The Egyptian Journal of Medical Human Genetics. 2017. Vol. 18, no. 2, pp.137-145.
https://search.emarefa.net/detail/BIM-760885
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 144-145
Record ID
BIM-760885