Cellular molecular changes in nerium oleander (L.)‎ cell culture under gamma radiation stress

Abstract EN

This study was done to analyze the relationship between the various effects of five different doses of gamma ray treatments (control, 0, 100, 200, 300 and 400 rad) on cell suspension culture of Nerium oleander belonging to the family Apocynaceae, Plant samples were collected from Egyptain flora.

The five treatments of the plants were characterized by analyzing variability in frozen biomass cell suspension culture of N.

oleander through SDS PAGE and peroxidase isozymes.

The electrophorogram showed a total of 36 bands of proteins with molecular weight ranging from 10 to 225 KDa.

The protein diversity analysis was done based on the presence or the absence of bands trhus interpreting their relevence.

The histogram analysis clearly showed a high degree of diversity along these five treatments of the plant.

The results of electrophoretic patterns of peroxidase isozymes that was extracted from frozen biomass cell suspension cultures after receiving the different gamma doses revealed remarkable molecular changes in all treatments.

These changes in peroxidase isozymes and protein bands indicate the effect of the different irradiation treatments on the gene expiration.