Inter simple sequence repeat DNA (ISSR) polymorphism utility in haploid nicotiana alata irradiated plants for finding markers associated with gamma irradiation and salinity
Joint Authors
al-Fiki, A. I.
al-Metabteb, G.
Adli, M.
Source
Arab Journal of Nuclear Sciences and Applications
Issue
Vol. 50, Issue 1 (31 Jan. 2017), pp.95-102, 8 p.
Publisher
The Egyptian Society of Nuclear Science and Applications
Publication Date
2017-01-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
Nicotiana alata is an ornamental plant.
It is a member of family Solanasea.
Tobacco (Nicotiana spp.) is one of the most important commercial crops in the world.
Wild Nicotiana species, as a store house of genes for several diseases and pests, in addition to genes for several important phytochemicals and quality traits which are not present in cultivated varieties.
Inter simple sequence repeat DNA (ISSR) analysis was used to determine the degree of genetic variation in treated haploid Nicotiana alata plants.
Total genomic DNAs from different treated haploid plantlets were amplified using five specific primers.
All primers were polymorphic.
A total of 209 bands were amplified of which 135 (59.47%) polymorphic across the radiation treatments.
Whilst, the level of polymorphism among the salinity treatments were 181 (85.6%).
Whereas, the polymorphism among the combined effects between gamma radiation doses and salinity concentrations were 283 (73.95%).
Treatments relationships were estimated through cluster analysis (UPGMA) based on ISSR data.
American Psychological Association (APA)
al-Fiki, A. I.& Adli, M.& al-Metabteb, G.. 2017. Inter simple sequence repeat DNA (ISSR) polymorphism utility in haploid nicotiana alata irradiated plants for finding markers associated with gamma irradiation and salinity. Arab Journal of Nuclear Sciences and Applications،Vol. 50, no. 1, pp.95-102.
https://search.emarefa.net/detail/BIM-761003
Modern Language Association (MLA)
al-Fiki, A. I.…[et al.]. Inter simple sequence repeat DNA (ISSR) polymorphism utility in haploid nicotiana alata irradiated plants for finding markers associated with gamma irradiation and salinity. Arab Journal of Nuclear Sciences and Applications Vol. 50, no. 1 (Jan. 2017), pp.95-102.
https://search.emarefa.net/detail/BIM-761003
American Medical Association (AMA)
al-Fiki, A. I.& Adli, M.& al-Metabteb, G.. Inter simple sequence repeat DNA (ISSR) polymorphism utility in haploid nicotiana alata irradiated plants for finding markers associated with gamma irradiation and salinity. Arab Journal of Nuclear Sciences and Applications. 2017. Vol. 50, no. 1, pp.95-102.
https://search.emarefa.net/detail/BIM-761003
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 101-102
Record ID
BIM-761003