Effect of long-term administration of melatonin, vitamin E, vitamin C and their combinations on some lipid profiles and renal function tests in rats exposed to lead toxicity

Abstract EN

Lead (Pb) is a natural element and widespread in the environment.

Lead poisoning affect numerous organ systems.

This study was carried out to investigate long-term administration of melatonin, vitamin C, vitamin E and their combinations, on some serum biochemical parameters in Pb-induced toxicity.

Fifty-four female albino rats were used in this study.

Animals were divided into nine groups.

The groups are: control, sodium acetate (0.1mg/L drinking water), Pb-acetate (0.1 mg/L in drinking water), Pb-acetae+melatonin (60 mg of melatonin/Kg diet), Pb-acetate+vitamin E (1000 IU of vitamin E/Kg diet), Pb-acetae+vitamin C (1mg of vitamin C/ L in drinking water), combinations: Pb-acetae+melatonin+vitamin E, Pb-acetae+melatonin+vitamin C, and Pb-acetate+vitamin E+vitamin C.

Results show significant increases in serum total cholesterol (TC) and triacylglycerol (TAG) in Pb-acetae group compared to the control rats.

Administration of melatonin, vitamin E, melatonin+vitamin E and vitamins C+E combinations caused significant reductions in serum TC and TAG, while, serum total protein increased significantly in melatonin and melatonin+vitamin E groups compared with Pb-acetate treated rats.

Serum creatinine, urea and uric acid exhibited significant increases in Pb-acetae treated rats in comparision with controls.

On the other hand, melatonin, melatonin+vitamin E combination decreased serum creatinine, urea, uric acid, and total bilirubin in comparision with Pb-acetate treated rats.

In conclusion, long-term lead treated rats exhibited marked elevation of serum TC,TAG, creatinine, urea, uric acid and total bilirubin levels.

Most of these abnormal changes were ameliorated with melatonin+ vitamin E combination much more than with melatonin, vitamin E or C alone or vitamins E and C in combination.