Bacteriological study and antibacterial activity of honey against some pathogenic bacteria isolated from burn infections

Abstract EN

This study was conducted at Emergency Management Center (EMC) in Erbil city to analyze (Isolation and Identification) the bacterial isolates from infected burn wounds of patients admitted to the burns unit and to determine the sensitivity pattern of the cultured bacteria to some commonly antibiotics and different honeys as antibacterial agent.

A total of 50 samples (surface swab) were analyzed, 45 positive samples yielding 73 isolates, of which 59 (80.82%) were Gram-negative bacteria and 14 (19.18%) Gram-positive bacteria.

The mean age was 21.51 years (range: 1-45 years) and infection was most prevalent in age group 21-25 (37.77%).

Among the patients, 32 (71.11%) were female and 13 (28.89%) male with highest percentage of staying in the hospital occur for (6-10) days.

Flame 35 (77.78%) was the most common cause of burn injuries and 25 (55.56%) of a total patients had third degree burns.

The most common bacterial isolates were Pseudomonas aeruginosa 33 (45.21%), Klebsiella pneumoniae 23 (31.51%), Staphylococcus aureus 11 (15.06%), Staphylococcus epidermidis 3 (4.11%), Proteus mirabilis 2 (2.74%) and Escherichia coli 1 (1.37%).

The results of the antibiotics susceptibility showed that Gram-negative bacteria were highly sensitive to Amikacin 76.65%, Chloramphenicol 75.79%, Gentamicin 69.70% and Ciprofloxacin 68.87% while Gram-positive bacteria were more sensitive to Vancomycin 100% and Clindamycin 90.90%.

Different honey samples investigated for their in vitro antibacterial activity against antibiotic resistant bacterial isolates showed that excellent antibacterial activity against Gram-negative and Gram-positive bacteria at 20% concentration for Qandel and Peshtashan honey (except K.

pneumoniae and S.

aureus, the MIC was at 30% concentration of Peshtashan honey) while 30% concentration for Sunbulah honey.