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Identification of single nucleotide polymorphisms in growth hormone secretagogue receptor (GHSR) gene in native Egyptian chickens
Joint Authors
Abu al-Majd, Muhammad
Sharaf, Muhammad M.
Radwan, Muhammad A.
Ahmad, Hana A.
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 12, Issue 1 (30 Jun. 2014), pp.127-138, 12 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2014-06-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Growth hormone secretagogue receptor (GHSR) or Ghrelin receptor gene is legend for a peptide hormone produced by chicken proventriculus which stimulates growth hormone release and food intake.
The purpose of this study was to determine single nucleotide polymorphisms (SNPs) in GHSR gene in six native Egyptian chicken breeds (Fayomi, Dandarawy, El-Salam, Dokki-4, Inshas, and Mandara) compared to Rhode Island Red (RIR).
Blood samples were collected from these chickens at eight weeks of age.
A PCR product of 598 bp, GHSR was amplified using polymerase chain reaction (PCR) and subsequently, subjected to sequence analysis to identify its different allelic patterns.
Two different SSCP banding patterns (TT and TC) were detected.
Nucleotide sequencing revealed presence of a novel T40C SNP in the first intron of GHSR at nucleotide number 59 before the second exon.
Results from SSCP and sequencing denoted absence of CC genotype in all examined chicken breed.
Although, we did not perform association analysis for these genotypes due to small population size, the present results put the basis for further studies to associate this SNP and others in GHSR with growth traits in local chicken breeds
American Psychological Association (APA)
Radwan, Muhammad A.& Abu al-Majd, Muhammad& Sharaf, Muhammad M.& Ahmad, Hana A.. 2014. Identification of single nucleotide polymorphisms in growth hormone secretagogue receptor (GHSR) gene in native Egyptian chickens. Kafr El-Sheikh Veterinary Medical Journal،Vol. 12, no. 1, pp.127-138.
https://search.emarefa.net/detail/BIM-761343
Modern Language Association (MLA)
Radwan, Muhammad A.…[et al.]. Identification of single nucleotide polymorphisms in growth hormone secretagogue receptor (GHSR) gene in native Egyptian chickens. Kafr El-Sheikh Veterinary Medical Journal Vol. 12, no. 1 (2014), pp.127-138.
https://search.emarefa.net/detail/BIM-761343
American Medical Association (AMA)
Radwan, Muhammad A.& Abu al-Majd, Muhammad& Sharaf, Muhammad M.& Ahmad, Hana A.. Identification of single nucleotide polymorphisms in growth hormone secretagogue receptor (GHSR) gene in native Egyptian chickens. Kafr El-Sheikh Veterinary Medical Journal. 2014. Vol. 12, no. 1, pp.127-138.
https://search.emarefa.net/detail/BIM-761343
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 136-138
Record ID
BIM-761343