Identification of novel SNP in prolactin promoter and its association with milk traits in be hairy Egyptian water buffaloes

Abstract EN

Prolactin hormone (PRL) plays a crucial role in the initiation and maintenance of lactation in the animals.

It is a potential quantitative trait locus and genetic marker of production traits in dairy cattle.

However, no SNP was detected and no association studies was conducted on PRL gene in Egyptian buffalo up till now.

Therefore, the objective of the present study was planned to detect PRL gene polymorphisms in Behairy Egyptian water buffalo.

A PCR product of 245 bp, including part of the promoter of PRL was amplified using PCR and subsequently, SSCP and nucleotide sequencing were conducted to identify its different allelic patterns in Behairy Egyptian water buffaloes.

Our results revealed the presence of a novel T72C SNP in the PRL promoter.

Moreover, two genotypes (TC, CC) were identified using SSCP technique.

These genotypes did not show any association with milk traits (milk yield, fat%, protein%, lactose%, total solid%) in the animals under the study.

We conclude that there were no significant difference in milk traits between TC and CC genotypes in Behairy Egyptian water buffaloes and hence the breeders should not depend on this SNP in their selection for dairy animals