A novel snp in exon 5 of the ghr gene and its association with average daily gain in beheiry egyptian water buffaloes (Bubalus Bubalis)‎

Abstract EN

The polymorphisms in growth hormone receptor gene (GHR) were determined by advanced molecular techniques as well as their association with growth traits in Beheiry Egyptian water buffalo.

Therefore, the objective of this study was to detect GHR gene polymorphisms in Beheiry Egyptian water buffalo.

Our results revealed the presence of SNP C742T in Beheiry Egyptian water buffalo by using PCR and SSCP methods followed by nucleotide sequencing to identify its different allelic patterns in the same animals under study.

Three genotypes (CC, TC and TT) were identified by SSCP technique.

Statistical analysis showed that, genotype TT was significantly higher than CT and CCgenotypes at age from 6 to 12 month (P < 0.05).

These genotypes exhibited an association with growth traits (body weight, average daily gain) in Beheiry Egyptian water buffaloes.

We could concluded that ther were a significant differences in growth traits between Beheiry Egyptian water buffaloes (TT, TC and CC genotypes) and so the breeders must take in consideration the presence of this SNP as well as its association with growth traits in the selection of Egyptian water buffalo for breeding.