Isolation and sequencing of myf5 gene (promoter region)‎ from Beheiry Egyptian water buffaloes

Abstract EN

Myogenic factor 5 (Myf5) is a member in the myogenic regulatory factor (MRF) family of basic helix-loop-helix transcription factors, which is involved in the differentiation and maturation of myotubes and is highly expressed postnatally.

The sequences of this gene are well known in cattle and small ruminants but in buffalo no available sequences were previously recorded.

Therefore, the current investigation threw the light on the isolation and sequencing the promoter region of this gene, which is the most appropriate portion of Myf5 that contains single nucleotide polymorphisms (SNPs), in Egyptian buffalo.

To achieve this task, PCR was performed in which gene-specific primers flanking to promoter region of Myf5 were utilized.

A PCR products of 700bp, of Myf5 gene corresponding to the expected product size, was successfully amplified.

Sequencing of the purified PCR products.

A novel A309T SNP was detected in the promoter sequences of the Myf5 locus (at nucleotide number 309 of the promoter) among Beheiry Egyptian water buffaloes.

Three different single-stranded conformation polymorphism patterns were observed in the Myf5 locus: TT, AT, and AA with frequencies of 0.26, 0.23 and 0.51 respectively.

Statistical analysis revealed that, the homozygous TT genotype was significantly associated with the average daily gain than AT and AA genotypes from birth to 9 mo of age.

The results of this study could be used as a basis for further investigations to associate the detected SNPs with meat quality traits in Egyptian water buffaloes.