Investigation of salmonella enteritidis colonization in the reproductive tract, forming EGG, freshly laid eggs of naturally infected ducks

Abstract EN

Salmonella enterica serovar enteritidis the ″hot strain″ infected the ovary, intestine, forming egg of naturally infected ducks proved to have unique DNA allele by ERIC-PCR.

The present study sought to determine whether several Salmonella Enteritidisisolates obtained from forming egg associated with reproductive tissue by naturally infected duckshad unique DNA allele or not? Ninety five ducks fromdifferent three farms in this study (thirty six from farm one, thirty one from farm two and twenty eight from farm three) and 135 freshly laid eggs collected from the same farms.Nine ducksout of theninety fiveducks were positive for detection of Salmonella Enteritidis bacteriologically, typed serologically and confirmed with MultiplexPCR on gene OMPC (204 bp) for genus Salmonella and gene ENT (304 bp) for Salmonella serotype Enteritidis.

Salmonella Enteritidiswasdetectedin ovaries; oviduct, forming eggs and intestine of one duck were 4, 2, 2 and 4cases respectively out of 95ducks tissue organs.

Salmonella Enteritidis in outer eggs shell was 3 and 0 in internal eggs contents out of 135 freshly laid eggs of the same farms