Induction of glucose-fructose oxidoreductase enzyme production using gamma-irradiated zymomonas mobilis

Abstract EN

In the present study, from three distinctive production media were used, media III was selected as it gave the highest production of bio-products under study.

The effect of different inoculum sizes (2.0, 4.0, 6.0, and 8.0g/l) on the production of the selected media were studied and the results indicated that 2.0g/l was the best inoculum size.

The effects of important independent factors on the fermentation of simultaneous production of glucose –fructose oxidoreductase (GFOR) enzyme, sorbitol, and gluconic acid by Zymomonas mobilis grown on the selected media components with different concentrations, time , pH, using a Plackett–Burman (P–B) design has been studied.

The results suggested that, yeast extract, peptone, concentration of carbon and pH were affected positively on the fermentation process; meanwhile, culture time was affected negatively on it.

The irradiation dose level of 50 Gy was the best from different tested doses of gamma irradiation (20Gy-1.0 kGy) for the fermentation process.

The effect of different wastes acid hydrolyzates, which were used as a sole carbon source or as a mixture with the basic synthetic media, on irradiated Z.

mobilis (50 Gy) has been determined.

The hydrolyzed extract of carob pod, orange and banana peels, showed a higher yield of bio-products compared with rice straw hydrolyzed extract