Investigate the relation between polymorphism of IL-13 gene and asthma at Thi-Qar province Iraq

Abstract EN

The current study was conducted at the Al-Hussein Teaching Hospital in Thi- Qar province, during the period from October 2014 to May 2015.

The study aimed to investigate polymorphism of IL-13 gene in patients with Asthma.

The study included a total of 100 patients with asthma (32 males) and (68 females) and there aged between 17-62 years, and 50 individual healthycontrol.

DNA was isolated and PCR was performed using primers specific for the IL-13 gene, the results showed the presence of mutations in a sample of 48 out of 100 patients with asthma at site 1112C/T after using restriction enzyme BstUI.

The results of the statistical analysis showed correlation T) in the /between the occurrence of the disease and the emergence of mutation (C promoter region of IL-13 gene when compared with the healthy control in population of Thi-qar province.