The effect of leonuri herba alkaloids on senile BPH (male and female hormone induced)‎ model rats

Abstract EN

Objective: To investigate the pharmacological effects of Leonuri Herba alkaloids (LHA) on prostate hyperplasia in older rats and the effect mechanism.

Methods: Remove bilateral testes from BPH model rats, and conduct subcutaneous injection of testosterone and estradiol.

At the same time, feed corresponding drugs to the rats by gastric perfusion for 30d.

In the first 27d, conduct bladder fistula surgery.

Three days after feeding, carry out the detection of the urine flow dynamics.

Eyeball blood taking, determination of serum E2 levels, and quickly remove the prostate, thymus gland, spleen, kidney, lung, and bladder.

1/3 prostate homogenate, determine the level of PACP, T, DHT.

1/3 prostate was determined by mRNA expression in bFGF.

The remaining 1/3 prostate was observed by light microscopy.

Results: LHA could significantly decrease the animal prostate index, level of DHT, T, PACP, and elevate levels of E2 in the serum.

It could also significantly reduce the maximum voiding pressure, intercontraction interval, and bladder resting pressure.

Conclusion: LHA has good therapeutic effect on prostatic hyperplasia model rats induced by male and female hormone.