Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase

Joint Authors

Wang, Yuzhu
Zhu, Xuemin
Liu, Zongzheng
Deng, Wen
Zhang, Ziqiang
Liu, Yumei
Wei, Lan
Zhang, Yuling
Zhou, Liutao

Source

Saudi Journal of Biological Sciences

Issue

Vol. 24, Issue 3 (31 Mar. 2017), pp.519-525, 7 p.

Publisher

Saudi Biological Society

Publication Date

2017-03-31

Country of Publication

Saudi Arabia

No. of Pages

7

Main Subjects

Biology

Topics

Abstract EN

Bone marrow mesenchymal stem cells (BMSCs) are a type of adult stem cells with a wide range of potential applications.

However, BMSCs have a limited life cycle under normal culturing conditions, which has hindered further study and application.

Many studies have confirmed that cells modified by telomerase reverse transcriptase (TERT) can maintain the ability to proliferate in vitro over a long period of time.

In this study, we constructed a gene expression vector to transfer TERT into sheep BMSCs, and evaluated whether the TERT cell strain was successfully transferred.

The abilities of cell proliferation and differentiation were evaluated using the methods including growth curve determination, inheritance stability analysis, multi-directional induction and so on, and the results showed that the cell strain can be cultured to 40 generations, with a normal karyotype rate maintained at 88.24%, and that the cell strain can be transferred and differentiated into neurocytes and lipocytes, proving that it retains the multi-directional transdifferentiation ability.

American Psychological Association (APA)

Zhu, Xuemin& Liu, Zongzheng& Deng, Wen& Zhang, Ziqiang& Liu, Yumei& Wei, Lan…[et al.]. 2017. Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase. Saudi Journal of Biological Sciences،Vol. 24, no. 3, pp.519-525.
https://search.emarefa.net/detail/BIM-761759

Modern Language Association (MLA)

Zhu, Xuemin…[et al.]. Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase. Saudi Journal of Biological Sciences Vol. 24, no. 3 (Mar. 2017), pp.519-525.
https://search.emarefa.net/detail/BIM-761759

American Medical Association (AMA)

Zhu, Xuemin& Liu, Zongzheng& Deng, Wen& Zhang, Ziqiang& Liu, Yumei& Wei, Lan…[et al.]. Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 3, pp.519-525.
https://search.emarefa.net/detail/BIM-761759

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 524-525

Record ID

BIM-761759