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Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase
Joint Authors
Wang, Yuzhu
Zhu, Xuemin
Liu, Zongzheng
Deng, Wen
Zhang, Ziqiang
Liu, Yumei
Wei, Lan
Zhang, Yuling
Zhou, Liutao
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 3 (31 Mar. 2017), pp.519-525, 7 p.
Publisher
Publication Date
2017-03-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Bone marrow mesenchymal stem cells (BMSCs) are a type of adult stem cells with a wide range of potential applications.
However, BMSCs have a limited life cycle under normal culturing conditions, which has hindered further study and application.
Many studies have confirmed that cells modified by telomerase reverse transcriptase (TERT) can maintain the ability to proliferate in vitro over a long period of time.
In this study, we constructed a gene expression vector to transfer TERT into sheep BMSCs, and evaluated whether the TERT cell strain was successfully transferred.
The abilities of cell proliferation and differentiation were evaluated using the methods including growth curve determination, inheritance stability analysis, multi-directional induction and so on, and the results showed that the cell strain can be cultured to 40 generations, with a normal karyotype rate maintained at 88.24%, and that the cell strain can be transferred and differentiated into neurocytes and lipocytes, proving that it retains the multi-directional transdifferentiation ability.
American Psychological Association (APA)
Zhu, Xuemin& Liu, Zongzheng& Deng, Wen& Zhang, Ziqiang& Liu, Yumei& Wei, Lan…[et al.]. 2017. Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase. Saudi Journal of Biological Sciences،Vol. 24, no. 3, pp.519-525.
https://search.emarefa.net/detail/BIM-761759
Modern Language Association (MLA)
Zhu, Xuemin…[et al.]. Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase. Saudi Journal of Biological Sciences Vol. 24, no. 3 (Mar. 2017), pp.519-525.
https://search.emarefa.net/detail/BIM-761759
American Medical Association (AMA)
Zhu, Xuemin& Liu, Zongzheng& Deng, Wen& Zhang, Ziqiang& Liu, Yumei& Wei, Lan…[et al.]. Derivation and characterization of sheep bone marrow-derived mesenchymal stem cells induced with telomerase reverse transcriptase. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 3, pp.519-525.
https://search.emarefa.net/detail/BIM-761759
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 524-525
Record ID
BIM-761759