Optimizing conditions for calcium phosphate mediated transient transfection
Joint Authors
Qi, Zhang
Ling, Guo
Liyang, Wang
Ronghua, Yang
Rui, Feng
Zhongguang, Li
Xin, Zhou
Zhilong, Dong
Ghartey Kwansah, George
Xu, Meng Meng
Nishi, Miyuki
Isaacs, Williams
Ma, Jianjie
Xuehong, Xu
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 3 (31 Mar. 2017), pp.622-629, 8 p.
Publisher
Publication Date
2017-03-31
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Topics
Abstract EN
Background: Calcium phosphate mediated transfection has been used for delivering DNA into mammalian cells in excess of 30 years due to its most low cost for introducing recombinant DNA into culture cells.
However, multiple factors affecting the transfect efficiency are commonly recognized meanwhile for years, the low transfection efficiency of this approach on higher differentiated and non-tumor cells such as CHO and C2C12 limits its application on research.
Results: In this paper, we systematically evaluated the possible factors affecting the transfection rate of this approach.
Two categories, calcium phosphate–DNA co-precipitation and on-cell treatments were set for optimization of plasmid DNA transfection into CHO and C2C12 cell-lines.
Throughout experimentation of these categories such as buffer system, transfection media and time, glycerol shocking and so on, we optimized the best procedure to obtain the highest efficiency ultimately.
During calcium phosphate DNA-precipitation, the transfection buffer is critical condition optimized with HBS at pH 7.10 (P = 0.013 compared to HEPES in CHO).
In the transfection step.
American Psychological Association (APA)
Ling, Guo& Liyang, Wang& Ronghua, Yang& Rui, Feng& Zhongguang, Li& Xin, Zhou…[et al.]. 2017. Optimizing conditions for calcium phosphate mediated transient transfection. Saudi Journal of Biological Sciences،Vol. 24, no. 3, pp.622-629.
https://search.emarefa.net/detail/BIM-761772
Modern Language Association (MLA)
Ling, Guo…[et al.]. Optimizing conditions for calcium phosphate mediated transient transfection. Saudi Journal of Biological Sciences Vol. 24, no. 3 (Mar. 2017), pp.622-629.
https://search.emarefa.net/detail/BIM-761772
American Medical Association (AMA)
Ling, Guo& Liyang, Wang& Ronghua, Yang& Rui, Feng& Zhongguang, Li& Xin, Zhou…[et al.]. Optimizing conditions for calcium phosphate mediated transient transfection. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 3, pp.622-629.
https://search.emarefa.net/detail/BIM-761772
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 629
Record ID
BIM-761772