Oridonin effectively reverses the drug resistance of cisplatin involving induction of cell apoptosis and inhibition of MMP expression in human acute myeloid leukemia cells
Joint Authors
Zhang, Yuan
Huang, Yan
Zi, Youmei
Zhang, Lingxiu
Wang, Lihua
Guo, Yan
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 3 (31 Mar. 2017), pp.678-686, 9 p.
Publisher
Publication Date
2017-03-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
Cisplatin is the first generation platinum-based chemotherapy agent.
However, the extensive application of cisplatin inevitably causes drug resistance, which is a major obstacle to cancer chemotherapy.
Oridonin is a diterpenoid isolated from Rabdosia rubescens with potent anticancer activity.
The aim of our study is to investigate the role of oridonin to reverse the cisplatinresistance in human acute myeloid leukemia (AML) cells.
The effect of oridonin on human AML cell proliferation was evaluated by MTT assay, cell migration and invasion were evaluated by transwell migration and invasion assays in cisplatin-resistant human AML cells.
Furthermore, cell apoptosis was examined by flow cytometry.
The inhibitive effect of oridonin in vivo was determined using xenografted nude mice.
In addition, the expressions of MMP2 and MMP9 were detected by Western blot.
There was a synergistic antitumor effect between cisplatin and oridonin on cisplatin-resistant human AML cells in vitro and in vivo.
In addition, the combination of cisplatin and oridonin synergistically induced cell apoptosis.
Furthermore, the combination treatment not only inhibited AML cell migration and invasion, but more significantly, decreased the expressions of MMP2 and MMP9 proteins.
Our results suggest that the synergistic effect between both agents is likely to be driven by the inhibition of MMP expression and the resulting increased apoptosis.
American Psychological Association (APA)
Zhang, Yuan& Wang, Lihua& Zi, Youmei& Zhang, Lingxiu& Guo, Yan& Huang, Yan. 2017. Oridonin effectively reverses the drug resistance of cisplatin involving induction of cell apoptosis and inhibition of MMP expression in human acute myeloid leukemia cells. Saudi Journal of Biological Sciences،Vol. 24, no. 3, pp.678-686.
https://search.emarefa.net/detail/BIM-761782
Modern Language Association (MLA)
Zhang, Yuan…[et al.]. Oridonin effectively reverses the drug resistance of cisplatin involving induction of cell apoptosis and inhibition of MMP expression in human acute myeloid leukemia cells. Saudi Journal of Biological Sciences Vol. 24, no. 3 (Mar. 2017), pp.678-686.
https://search.emarefa.net/detail/BIM-761782
American Medical Association (AMA)
Zhang, Yuan& Wang, Lihua& Zi, Youmei& Zhang, Lingxiu& Guo, Yan& Huang, Yan. Oridonin effectively reverses the drug resistance of cisplatin involving induction of cell apoptosis and inhibition of MMP expression in human acute myeloid leukemia cells. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 3, pp.678-686.
https://search.emarefa.net/detail/BIM-761782
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 685-686
Record ID
BIM-761782