Modulation male fertility in diabetic rats by Allicin administration

Abstract EN

Diabetes has been known to cause fertility complications including male reproductive organs failure and infertility.

However, many studies have examined the anti-diabetic effect of major component of garlic compounds, allicin, in both types of diabetes mellitus (DM), but to date, the role of allicin in male fertility protection throughout disease process of diabetes mellitus remains unclear.

Objective: Hence this study has been undertaken to evaluate histological changes via allicin administration in the maintenance male reproductive system function in rats-exposed to diabetes mellitus.

Methods: Thirty adult male rats have been divided into three groups (10 rats in each group).

The two control groups, including diabetic rats (DM induced by intraperitoneal injection with a single dose of Streptozocin (STZ) 65mg/kg body weight) and normal rats, which were injected with normal saline, once daily for 21 days.

The third group received allicin.

Blood glucose levels were tested every day through 21 days of the experimental period.

At the end of the experiment, testis tissues were taken from the rats for histological examination and they were fixed in 10% neutral formalin solution and hematoxylin and eosin (H&E) stained sections of testis were prepared for histopathological and Histomorphometric examination under light microscope.

Results: The results were compared to the ones obtained from healthy and non-treated diabetic rats as well as allicin -treated diabetic rats.

Histopathological examinations of stained testicular tissue sections showed necrosis and vacuolization of spermatocytes to be reasonably reduced in the diabetic treated rats as well as demonstrated abnormal spermatogenises but it was found to restore the testicular histology and components of spermatogenises to near normal in the allicin treated group.

Subsequent histomorphometrical evaluation also showed a reduction in the diameter and height epithelia of seminiferous tubules in the diabetic control rats which were significantly improved in the allicin- treated diabetic rats (P < 0.05) which were comparable to normal rats.

Conclusion these results indicate that the active compound of garlic plant may help in the prevention of the complications of diabetes and confer essential protection for male reproductive function as serious complication.