Genetic diversity among walnuts (juglans regia)‎ population in Kurdistan Region-Iraq using AFLP-PCR

Abstract EN

In this study, the genetic relatedness of five walnut populations in five different locations: Bedohe (Kanimase), kanizarke (Akre), kuzo (Zawita) , Kashane (Batifa) and Sharaneshe (Darkare) in Duhok Governorate-Kurdistan region/Iraq was analyzed using fifteen AFLP primers pairs.

Similarity matrices were obtained based on the AFLP data to analyze genetic distance.

Genetic diversity coefficient ranged from 0.1075 to 0.2540.

The lowest genetic similarity was detected between sharansh and Akri (0.1075).

The kanemas and Akri populations were most similar ones with coefficient of 0.2540.

Clustering based on AFLP data for the five walnuts populations was identified at the 0.21 similarity level and two main clusters were identified, Cluster one included three populations: Akri, sharansh and bedoh; cluster two was composed of two populations: kanemas and Kuzo.

Total generated bands screened from 15 primers were 517 bands for the five walnut populations with an average of 34.46 per primer, which 294 were polymorphic bands with an average of 19.6 polymorphic loci per primer.