Evaluation of some histochemical and immunohistochemical criteria of round cell tumors of bone
Joint Authors
Numan, Hasan H.
Karrush, Sana Sulayman Abd al-Hamid
al-Badawi, Zaynab H.
Muhammad, Iman M. S.
Source
Journal of the Arab Society for Medical Research
Issue
Vol. 7, Issue 1 (30 Jun. 2012), pp.21-32, 12 p.
Publisher
Arab Society for Medical Research
Publication Date
2012-06-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Background / aims :The differential diagnosis of round cell tumors of bone (RCTB), Ewing sarcoma, smallcell osteosarcoma, mesenchymal chondrosarcoma, osteoblastoma, chondroblastoma, primary bone lymphoma, and multiple myeloma still remains a challenge.
Given the significant differences in treatment, an accurate diagnosis is crucial.
This study aimed to evaluate some histochemical and immunohistochemical criteria of RCTB. Participants and methods Periodic acid-Schiff (PAS), CD99, CD138, osteocalcin, and leukocyte common antigen (LCA) were evaluated in 113 patients with RCTB. Results PAS was positive in neoplastic cells of all Ewing sarcomas, 27% of osteosarcomas, 92% of chondrosarcomas, all osteoblastomas and chondroblastomas, and the osteoid tissue of all osteosarcomas and osteoblastomas.
CD99 was positive in all Ewing sarcomas, in 11, 4, and 11% of osteoblastomas, multiple myelomas, and bone lymphomas, respectively.
CD99 was higher in Ewing sarcoma than in other RCTB (Po0.0001).
Osteocalcin was positive in neoplastic cells of all osteosarcomas, osteoblastomas, and 20% of chondroblastomas, 84, and 78% of osteoid of osteosarcomas and osteoblastomas, respectively.
CD138 was positive in all multiple myelomas, 12% of Ewing sarcomas, 20% of osteosarcomas, 44% of osteoblastomas, 8% of chondrosarcomas, and 40% of chondroblastomas.
CD138 was higher in multiple myeloma (Po0.0001) than in other RCTB.
LCA positivity was higher (Po0.01) in bone lymphomas (100%) than in multiple myelomas (73%). Conclusion PAS negativity excludes multiple myeloma and bone lymphoma from other RCTB that could be differentiated by LCA and CD138.
CD99 positivity confirms the diagnosis of Ewing sarcoma.
PAS could detect areas of osteoid in osteosarcoma and osteoblastoma.
Osteocalcin suggests an osteogenic tumor origin: osteosarcoma/ osteoblastoma.
Double negativity of CD99 and osteocalcin suggests a chondrogenic tumor origin: chondrosarcoma/chondroblastoma.
American Psychological Association (APA)
Muhammad, Iman M. S.& al-Badawi, Zaynab H.& Karrush, Sana Sulayman Abd al-Hamid& Numan, Hasan H.. 2012. Evaluation of some histochemical and immunohistochemical criteria of round cell tumors of bone. Journal of the Arab Society for Medical Research،Vol. 7, no. 1, pp.21-32.
https://search.emarefa.net/detail/BIM-762227
Modern Language Association (MLA)
Muhammad, Iman M. S.…[et al.]. Evaluation of some histochemical and immunohistochemical criteria of round cell tumors of bone. Journal of the Arab Society for Medical Research Vol. 7, no. 1 (Jan. / Jun. 2012), pp.21-32.
https://search.emarefa.net/detail/BIM-762227
American Medical Association (AMA)
Muhammad, Iman M. S.& al-Badawi, Zaynab H.& Karrush, Sana Sulayman Abd al-Hamid& Numan, Hasan H.. Evaluation of some histochemical and immunohistochemical criteria of round cell tumors of bone. Journal of the Arab Society for Medical Research. 2012. Vol. 7, no. 1, pp.21-32.
https://search.emarefa.net/detail/BIM-762227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 31-32
Record ID
BIM-762227