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Callus induction and proliferation as affected by EMS and UV-B mutagens in rice (Oryza Sativa L.) genotypes
Other Title(s)
استحثاث الكالس و تكاثره متأثرا بالمطفرين EMS و UV-B لتركيبين وراثيين من الرز (Oryza sativa L.)
Joint Authors
Muhammad, Rasha Karim
Ibrahim, Kazim Muhammad
Source
Issue
Vol. 57, Issue (sB) (31 Dec. 2016), pp.296-303, 8 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Seeds of the two rice genotypes namely Amber 33 (A33) and Amber Baghdad (AB) were divided into two groups; the first was presoaked in different concentrations of ethyl methane sulphonate (EMS) as chemical mutagen for different duration times (3, 6 and 12) hrs, and the other was exposed to different exposure times of ultra violate (UV-B) radiation (280-320 nm) as physical mutagen for different times (20, 40 and 60) min at room temperature.
Treated and non-treated seeds were transferred into the callus induction medium containing 2.5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 mg/L benzyl adenine (BA) under aseptic conditions.
Calli were divided into two groups the first was treated with several EMS concentrations (0.0, 0.50.
1.0, 1.50 and 2.0) % for 30 min and the second group was irradiated with UV-B for several exposure times (20, 40 and 60) min to induce genetic variation.
Seeds were treated with the optimum dose that caused high % in callus induction.
The dose that caused 50% reduction in callus growth mass was adopted.
American Psychological Association (APA)
Muhammad, Rasha Karim& Ibrahim, Kazim Muhammad. 2016. Callus induction and proliferation as affected by EMS and UV-B mutagens in rice (Oryza Sativa L.) genotypes. Iraqi Journal of Science،Vol. 57, no. (sB), pp.296-303.
https://search.emarefa.net/detail/BIM-762586
Modern Language Association (MLA)
Muhammad, Rasha Karim& Ibrahim, Kazim Muhammad. Callus induction and proliferation as affected by EMS and UV-B mutagens in rice (Oryza Sativa L.) genotypes. Iraqi Journal of Science Vol. 57, no. (sB) (2016), pp.296-303.
https://search.emarefa.net/detail/BIM-762586
American Medical Association (AMA)
Muhammad, Rasha Karim& Ibrahim, Kazim Muhammad. Callus induction and proliferation as affected by EMS and UV-B mutagens in rice (Oryza Sativa L.) genotypes. Iraqi Journal of Science. 2016. Vol. 57, no. (sB), pp.296-303.
https://search.emarefa.net/detail/BIM-762586
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 302-303
Record ID
BIM-762586