تأثير البنزل ادنين و حامض السالسليك في النمو و إنتاج القلويدات الكلية لنبات الوذنية (Withania somnifera L.) خارج الجسم الحي
Other Title(s)
Effect of benzyl adenine and salicylic acid on growth and total alkaloids production of wethinia (Withania somnifera L.) in vitro
Joint Authors
Source
Issue
Vol. 48, Issue 1 (28 Feb. 2017), pp.256-265, 10 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2017-02-28
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract EN
An experiment was conducted in the tissue culture laboratory, College of Education For Pure Science, Diyala Uinversity in 2014-2015,to investigate the influences of Benzyl adenine and Salicylic acid on growth and total alkaloids production for Wethinia plant )Withania somnifera L.( in vitro .Four concentrations of Benzyl Adenine)BA( 0.0, 1, 2 and 3 mg.l-1, which were represented with A0 ، A1، A2 and A3 were used respectively، and also four concentrations of Salicylic acid (SA( 0, 10, 20, and 30 mg.l-1 which were represented with B0, B1,B2 and B3 respectively, with the interaction between of Benzyl adenine and Salicylic acid,these are concentrations added to culture media(MS) in multiplication stages.
Seeds were soaked with gibberellic acid 500 mg.L-1 for 18 h at room temperature.Benzyl adenine at 1 mg.L-1 significantly increased shoots number 4.22, and Chlrophyll content 34.07 SPAD Unit ,there was no significant differences at 2 mg.L-1 in leaves number 12.05,11.32, fresh and dry weight of vegetative parts 1.42,1.40 g, and 0.134,0.128 g, also the addition of SA at 10 mg.L-1 led to increased in number of shoots 3.45, number of leaves 14.05, dry weight of vegetative parts 0.140 g ,and fresh weight of root system 0.06 g, there was no significant differences at 20 mg.L-1 in Chlrophyll content 33.77, 35.47 SPAD Unit, fresh weight of vegetative parts,1.47, 1.40 g, number of roots 18.00,20.10, and percentage of rooting 7.89% ,8.51%.
Interaction between of Benzyl adenine and Salicylic acid (A1B1) showed a significant superiority in all studed vegetative characters, there was no significant differences with (A2B1) in leaves number15.30, 14.80 leaf, fresh weight of vegetative parts1.61,1.59 g, dry weight of vegetative parts 0.160 ,0.160 g, A0B1 treat ment which rooted and acclimated gave 0.105 mg.g-1 total alkaloids.Benzyl adenine at 2 mg.l-1 increased total alkaloids 0.091 mg g-1, Salicylic acid at 10 mg.l-1 increased total alkaloids0.091 mg.g-1,also Interaction of treatments A2B1 and A1B1 show a significant superiority of total alkaloids 0.101,0.100 mg.g-1as compared with A0B0 which gave lowest content 0.029 mg.g-1.
American Psychological Association (APA)
علي خلف حمود وبيان حمزة مجيد. 2017. تأثير البنزل ادنين و حامض السالسليك في النمو و إنتاج القلويدات الكلية لنبات الوذنية (Withania somnifera L.) خارج الجسم الحي. مجلة العلوم الزراعية العراقية،مج. 48، ع. 1، ص ص. 256-265.
https://search.emarefa.net/detail/BIM-763163
Modern Language Association (MLA)
علي خلف حمود وبيان حمزة مجيد. تأثير البنزل ادنين و حامض السالسليك في النمو و إنتاج القلويدات الكلية لنبات الوذنية (Withania somnifera L.) خارج الجسم الحي. مجلة العلوم الزراعية العراقية مج. 48، ع. 1 (2017)، ص ص. 256-265.
https://search.emarefa.net/detail/BIM-763163
American Medical Association (AMA)
علي خلف حمود وبيان حمزة مجيد. تأثير البنزل ادنين و حامض السالسليك في النمو و إنتاج القلويدات الكلية لنبات الوذنية (Withania somnifera L.) خارج الجسم الحي. مجلة العلوم الزراعية العراقية. 2017. مج. 48، ع. 1، ص ص. 256-265.
https://search.emarefa.net/detail/BIM-763163
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 264-265
Record ID
BIM-763163