BRAFV600 gene mutation in thyroid cancer in Duhok-Iraq
Other Title(s)
الكشف عن طفرة ال BRAF في حالات سرطان الغدة الدرقية بطريقة ال Real Time PCR و علاقتها بالتغيرات النسيجية
Joint Authors
Hassan, Nirmin Adlat
Pity, Intisar Salim
Salih, Ahmad Muhammad
Source
Issue
Vol. 9, Issue 2 (31 Dec. 2015), pp.74-86, 13 p.
Publisher
University of Duhok College of Medicine
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
Background and objective: This study was done to detect the frequency of BRAFV600 mutation in thyroid cancer in Duhok-Iraq, using real time PCR.
Methods: DNA was extracted from formalin fixed, paraffin embedded tissue sections taken from 54 surgically resected primary thyroid cancers.
Using Real-Time PCR, the target (BRAFV600) DNA was amplified with the mutation-specific primers.
Results: BRAFV600 gene mutation was detected in 27.8% of cases.
It was significantly high in conventional papillary carcinoma (26.7%) compared with other papillary variants (p= 0.048), and significantly high among cases with extrathyroid extension.
Both medullary and one anaplastic carcinomas were BRAFV600 positive.
In contrast, all follicular carcinomas and the follicular variant papillary carcinoma were negative for this gene mutation.
No association was found between this mutation and any of nodal involvement, gender and age.
Conclusions: Identification of BRAFV600 gene mutation in medullary carcinoma opposes the previous concept that this gene alteration is restricted to papillary carcinoma.
Lack of BRAFV600 gene mutation among all follicular variant PTC and follicular cancers including the Hurthel cell tumor, extended the argument that these tumors are closely related.
Because this mutation was detected in clinically aggressive cancers like MTC, columnar variant PTC and ATC and was restricted to microcarcinoma with nodal metastasis in addition to its significant association with extrathyroid extension (T4), BRAFV600 molecular testing might emerge as a clinically useful supplement for the histologic assessment as an important predictor of aggressive clinical course.
American Psychological Association (APA)
Pity, Intisar Salim& Salih, Ahmad Muhammad& Hassan, Nirmin Adlat. 2015. BRAFV600 gene mutation in thyroid cancer in Duhok-Iraq. Duhok Medical Journal،Vol. 9, no. 2, pp.74-86.
https://search.emarefa.net/detail/BIM-763751
Modern Language Association (MLA)
Pity, Intisar Salim…[et al.]. BRAFV600 gene mutation in thyroid cancer in Duhok-Iraq. Duhok Medical Journal Vol. 9, no. 2 (Dec. 2015), pp.74-86.
https://search.emarefa.net/detail/BIM-763751
American Medical Association (AMA)
Pity, Intisar Salim& Salih, Ahmad Muhammad& Hassan, Nirmin Adlat. BRAFV600 gene mutation in thyroid cancer in Duhok-Iraq. Duhok Medical Journal. 2015. Vol. 9, no. 2, pp.74-86.
https://search.emarefa.net/detail/BIM-763751
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 82-84
Record ID
BIM-763751