Ultra-structure study of microvilli, mitochondria and lipid droplets of Egyptian buffaloes’ oocytes with reference to quality

Other Title(s)

دراسة التركيب الدقيق للخملات و الميتوكندريا و حبيبات الدهن و علاقتها بجودة البويضات في الجاموس المصري باستخدام الميكروسكوب الإلكتروني

Joint Authors

al-Sayyid, Itidal H.
Ahmad, Daliyah Abd al-Rahman
Salim, Salim Muhammad
al-Sayyid, Ashraf
Barqawi, Ashraf Hisham

Source

Egyptian Journal of Animal Production

Issue

Vol. 49, Issue (sup) (30 Nov. 2012), pp.29-38, 10 p.

Publisher

Egyptian Society of Animal Production

Publication Date

2012-11-30

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Agriculture

Abstract EN

The objective of this study was to describe and relate the ultra-structure of the micro-villi and mitochondria of buffalo’ oocytes as well as morphological aspects of lipid droplets with particular reference to oocytes’ quality.

Ovaries were collected from local slaughterhouse and transported to the laboratory within three to four h post-slaughter.

Cumulus-oocyte complexes (COCs) were aspirated from antral follicles of two to eight mm diameter using an 18-guage needle.

A total number of 10 good and 10 denuded oocytes were selected and prepared to study the target structure using transmission electron microscopy.

Results indicated that good oocytes had large number (P<0.05) of micro-villi, compared to that of denuded ones.

In a few cases, micro-villi looked erected in good oocytes, while such cases were not clear in the denuded ones.

The mitochondria were located at the periphery of the oocyte and a small number were found close to the center with round, hooded or oval shaped.

The lipid droplets were found near to the mitochondria, they were small in size and number.

The distribution of organelles in the cytoplasm of oocytes markedly differed between good and denuded oocytes.

American Psychological Association (APA)

al-Sayyid, Ashraf& al-Sayyid, Itidal H.& Ahmad, Daliyah Abd al-Rahman& Salim, Salim Muhammad& Barqawi, Ashraf Hisham. 2012. Ultra-structure study of microvilli, mitochondria and lipid droplets of Egyptian buffaloes’ oocytes with reference to quality. Egyptian Journal of Animal Production،Vol. 49, no. (sup), pp.29-38.
https://search.emarefa.net/detail/BIM-764487

Modern Language Association (MLA)

Salim, Salim Muhammad…[et al.]. Ultra-structure study of microvilli, mitochondria and lipid droplets of Egyptian buffaloes’ oocytes with reference to quality. Egyptian Journal of Animal Production Vol. 49, no. (sup) (Nov. 2012), pp.29-38.
https://search.emarefa.net/detail/BIM-764487

American Medical Association (AMA)

al-Sayyid, Ashraf& al-Sayyid, Itidal H.& Ahmad, Daliyah Abd al-Rahman& Salim, Salim Muhammad& Barqawi, Ashraf Hisham. Ultra-structure study of microvilli, mitochondria and lipid droplets of Egyptian buffaloes’ oocytes with reference to quality. Egyptian Journal of Animal Production. 2012. Vol. 49, no. (sup), pp.29-38.
https://search.emarefa.net/detail/BIM-764487

Data Type

Journal Articles

Language

English

Notes

Includes appendices : p. 35-38

Record ID

BIM-764487