Shortening full-length aptamer by crawling base deletion-assisted by mfold web server application
Joint Authors
Gopinath, Subash C. B.
Lakshmipriy, Thangavel
Arshad, M. K. Md
Voon, C. H.
Adam, Tijjani
Hashim, Uda
Singh, Harbant
Chinni, Suresh V.
Source
Arab Journal of Basic and Applied Sciences
Issue
Vol. 23, Issue 0 (30 Jun. 2017), pp.37-42, 6 p.
Publisher
University of Bahrain College of Science
Publication Date
2017-06-30
Country of Publication
Bahrain
No. of Pages
6
Main Subjects
Topics
Abstract EN
Systematic Evolution of Ligands by EXponential enrichment (SELEX) is the method to select the specific aptamer against a wide range of targets.
For this process, the initial library usually has a length of random sequences from 5 and it reaches over 100 bases.
The lengthy sequences have disadvantages such as difficult to prepare, less stable and expensive.
It is wise to prefer shorter version of aptamer for a wide range of applications including drug delivery process.
It is a common practice to shorten the full-length aptamer by mapping analyses and it is tedious.
Here, we used a crawling method to shorten the aptamer by different sequential deletion of bases from both 50 and 30 ends, assisted by Mfold web server application.
Two different kinds of aptamer with varied lengths (randomized region of 30 and 74 bases) were desired for this study, generated against Influenza A/Panama/2007/1999 (H3N2) and gD protein of Herpes Simplex Virus-1.
It was found that shortening the aptamer length by crawling pattern is possible with the assistance of Mfold web server application.
The obtained results resemble the shortened aptamer derived by mapping analyses.
The proposed strategy is recommended to predict the shorter aptamer without involving any wet experimental section.
American Psychological Association (APA)
Gopinath, Subash C. B.& Lakshmipriy, Thangavel& Arshad, M. K. Md& Voon, C. H.& Adam, Tijjani& Hashim, Uda…[et al.]. 2017. Shortening full-length aptamer by crawling base deletion-assisted by mfold web server application. Arab Journal of Basic and Applied Sciences،Vol. 23, no. 0, pp.37-42.
https://search.emarefa.net/detail/BIM-766251
Modern Language Association (MLA)
Gopinath, Subash C. B.…[et al.]. Shortening full-length aptamer by crawling base deletion-assisted by mfold web server application. Arab Journal of Basic and Applied Sciences Vol. 23 (Jun. 2017), pp.37-42.
https://search.emarefa.net/detail/BIM-766251
American Medical Association (AMA)
Gopinath, Subash C. B.& Lakshmipriy, Thangavel& Arshad, M. K. Md& Voon, C. H.& Adam, Tijjani& Hashim, Uda…[et al.]. Shortening full-length aptamer by crawling base deletion-assisted by mfold web server application. Arab Journal of Basic and Applied Sciences. 2017. Vol. 23, no. 0, pp.37-42.
https://search.emarefa.net/detail/BIM-766251
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 42
Record ID
BIM-766251