HPLC method development, validation and its application to investigate in vitro effect of pioglitazone on the availability of H1 receptor antagonists
Joint Authors
Mirza, Agha Zeeshan
Sultana, Najmah
Arayne, M. Said
Source
Arab Journal of Basic and Applied Sciences
Issue
Vol. 22, Issue 0 (28 Feb. 2017), pp.70-75, 6 p.
Publisher
University of Bahrain College of Science
Publication Date
2017-02-28
Country of Publication
Bahrain
No. of Pages
6
Main Subjects
Topics
Abstract EN
The method has been developed and validated for the simultaneous determination of pioglitazone and H1-receptor antagonists (fexofenadine, cetirizine and levocetirizine) in formulations and human serum.
Utilizing HPLC techniques, an assay was designed to determine the in vitro effects of pioglitazone on H1-receptor antagonists.
Obtained results were verified using the UV spectrophotometric technique.
First-derivative values versus concentrations were used to plot calibration curves of these drugs and were found to similar with the HPLC data.
The availability of pioglitazone remained unchanged in absence or presence of fexofenadine, cetirizine and levocetirizine.
This in vitro analysis confirms the harmless co-administration of pioglitazone and H1- receptor antagonists, and can serve as the foundation for designing further in vivo studies.
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American Psychological Association (APA)
Mirza, Agha Zeeshan& Arayne, M. Said& Sultana, Najmah. 2017. HPLC method development, validation and its application to investigate in vitro effect of pioglitazone on the availability of H1 receptor antagonists. Arab Journal of Basic and Applied Sciences،Vol. 22, no. 0, pp.70-75.
https://search.emarefa.net/detail/BIM-766899
Modern Language Association (MLA)
Mirza, Agha Zeeshan…[et al.]. HPLC method development, validation and its application to investigate in vitro effect of pioglitazone on the availability of H1 receptor antagonists. Arab Journal of Basic and Applied Sciences Vol. 22 (Feb. 2017), pp.70-75.
https://search.emarefa.net/detail/BIM-766899
American Medical Association (AMA)
Mirza, Agha Zeeshan& Arayne, M. Said& Sultana, Najmah. HPLC method development, validation and its application to investigate in vitro effect of pioglitazone on the availability of H1 receptor antagonists. Arab Journal of Basic and Applied Sciences. 2017. Vol. 22, no. 0, pp.70-75.
https://search.emarefa.net/detail/BIM-766899
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 75
Record ID
BIM-766899